The neurotrophic or neurite-promoting effect of new compound, <beta> -citryl-L-glutamic acid on the primary cultured neuronal cells.

1.本文观察了-柠檬酰-L-谷氨酸（-CG）对培养7-10天的鸡胚大脑、小脑、视叶和睫状神经节神经细胞的神经营养或促神经突起生长作用。<beta><beta><beta>- CG对睫状神经节周围神经元细胞无影响，但对大脑、小脑、视叶等中枢神经元细胞，尤其是视叶神经元细胞有存活因子作用。- CG对视叶神经细胞的作用在20~200 μ M之间呈剂量-反应曲线.<beta><miero>原代培养视叶神经细胞9天，测定细胞内-CG含量.<beta><beta>- CG随着孵育时间的增加而急剧增加，至第6天，然后下降至第9天。在鸡胚发育过程中，视叶内-CG浓度的变化规律与发育过程中的变化规律相似.<beta>本文研究了鸡胚发育过程中大脑和视叶内-CG水解酶活性的发育变化。<beta>视叶的活性从孵育9到20逐渐增加，而大脑在孵育14时达到平台水平。<beta>从大鼠睾丸中分离纯化了β-CG水解酶。该蛋白分子量为180K，具有酸性糖蛋白性质，并与DNA具有亲和性。

1. The neurotrophic or neurite-promoting effect of <beta> -citryl-L-glutamic acid ( <beta> -CG) on the primary cultured neuronal cells from cerebrum, cerebellum, optic lobe or ciliary ganglion of chick embryo incubated for 7-10 days was examined. <beta> -CG had no effect on the peripheral neuronal cells from ciliary ganglion, but had the effect as survival factor on the central neuronal cells such as cerebrum, cerebellum and optic lobe, especially from optic lobe. The effect of <beta> -CG on the neuronal cells from optic lobe showed dose-responce curve between 20 and 200 <miero> M.2. The <beta> -CG content in the primary cultured neuronal cells from optic lobe was determined for 9 days. <beta> -CG increased extremely with the incubation time to day 6 and then decreased to day 9. The developmental change was similar to that of <beta> -CG concentration in the optic lobe during the growth of the chick embryo.3. The developmental change of <beta> -CG hydrolysing enzyme activity in the cerebrum and optic lobe was examined during the growth of chick embryo. The activity increased from incubation 9 to 20 in the optic lobe, but reached to the plateau level at incubation 14 in the cerebrum.<beta> -CG hydrolysing enzyme have been purified to the homogeneity from rat testes. This preparation had the molecular weight of 180 K and showed the nature as acidic glycoproteine and the affinity to DNA.