Analysis of immunological mechanisms for antibody production to hepatitis B surface antigen.

1. 抗独特型抗体增强乙型肝炎表面抗原抗体的产生。研究了抗乙型肝炎表面抗原抗体（anti-HBS）抗独特型抗体是否能调节人外周血单核细胞体外抗- hbs的产生。血清抗hbs阳性患者外周血单核细胞在加入抗- hbs的F <(ab')_2>片段和纯化的HBsAg后，与单独添加欧商草有丝分裂原培养的患者相比，产生的抗- hbs数量显著增加，这表明抗独特型抗体调节了对HBsAg的免疫反应。慢性乙型肝炎表面抗原（HBsAg）携带者和急性乙型肝炎患者外周血淋巴细胞体外合成乙型肝炎病毒抗原抗体的研究。用商陆有丝分裂原刺激培养慢性乙型肝炎表面抗原携带者、急性乙型肝炎（HAB）患者和血清中有抗乙型肝炎抗体（对照）的外周血单个核细胞，检测IgG、抗乙型肝炎抗体和抗乙型肝炎抗体的合成。共培养实验表明，慢性HBsAg携带者和AHB患者在恢复期血清中缺乏抗- hbs的原因是乙肝表面抗原特异性的B细胞功能下降和抑制性T细胞功能增加。白细胞介素2增强乙型肝炎表面抗原抗体的产生。我们研究了用美洲商陆有丝分裂原诱导培养淋巴细胞产生抗hbs是否可以用白细胞介素2 （IL2）代替。在用纯化的HBsAg和il - 2刺激的培养物中，当使用血清抗hbs阳性患者和HBsAg疫苗接受者的外周血单个核细胞作为效应细胞时，抗hbs的产生水平与单独用美洲商陆有丝分裂原刺激的培养物相当。然而，单独il - 2不能诱导抗- hbs的产生。

1. Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody. Studies were undertaken to determine whether anti-idiotypic antibody against antibody to hepatitis B surface antigen (anti-HBS) could modulate in vitro anti-HBS production by human peripheral blood mononuclear cells stimulated with pokeweed mitogen. Peripheral blood mononuclear cells from patients positive for serum anti-HBs produced significantly increased amounts of anti-HBs by the addition of F <(ab')_2> fragments of anti-anti-HBs as well as purified HBsAg when compared to those in cultures with pokeweed mitogen alone, indicating that anti-idiotypic antibody modulates the immune response to HBsAg.2. In vitro synthesis of antibody to hepatitis B virus antigen by circulating lymphocytes from chronic HBsAg carriers and patients with acute hepatitis B. The synthesis of IgG, anti-HBs and anti-HBc was measured in pokeweed mitogen-stimulated cultures of peripheral blood mononuclear cells from chronic HBsAg carriers, patients with acute hepatitis B (HAB) and subjects with anti-HBs in serum (controls). Coculture experiments indicated that decreased B cell function and increased suppressor T cell function specific for HBsAg contributed to the lack of anti-HBs in serum of chronic HBsAg carriers and AHB patients during the recovery phase.3. Enhancement of antibody production to hepatitis B surface antigen by interleukin 2. We studied to assess whether induction of anti-HBs production by cultured lymphocytes which had been conducted with pokeweed mitogen could be replaced by interleukin 2 (IL2). In cultures stimulated with purified HBsAg and IL2, the comparable levels of anti-HBs production to those obtained in cultures stimulated with pokeweed mitogen alone occured when peripheral blood mononuclear cells from patients positive for serum anti-HBs and recipients of HBsAg vaccine were used as effector cells. However, IL2 alone did not induce anti-HBs production.