Articifial Induction of cleavage furrow in cell division

1-甲基腺嘌呤处理后，将海星（Asterina pectinifera）的成熟卵母细胞转移到含有2.5%己二醇（HG）的海水中。约20-30分钟后，减数分裂器（MA）开始清晰可见，作为靠近细胞膜的一个小点，随着时间的推移，尺寸开始增加。在卵母细胞增大后，大部分较大的MA垂直或平行于细胞膜，但在少数卵母细胞的情况下靠近细胞中心。在HG处理的卵母细胞的中期，分离的MA的星状体的直径比对照实验中的长2 - 3倍。根据其在卵母细胞成熟过程中的特定位置，较大的MA诱导减数分裂过程中的三个不同的事件：巨大的极体形成，在大多数卵母细胞中的单边开沟，和平等的细胞分裂在少数卵母细胞。这些事件的发生频率取决于卵母细胞在成熟过程中转移到HG-SW的时间。基于这些发现，我们试图提出一个解释HG诱导的事件。当成熟卵母细胞被转移到HG SW中时，HG逐渐扩散到其中，使迁移的MA稳定在其成熟过程中的特定位置，并通过其周围微管的重组使其体积增大，较大的MA可能导致卵母细胞减数分裂的改变，导致随后的上述三个事件的诱导，这取决于成熟过程中卵母细胞的特定位置。

Following 1-methyladenine treatment, maturing oocytes of the starfish, Asterina pectinifera, were transferred to sea water containing 2.5% hexyleneglycol(HG). After about 20-30 min, meiotic apparatus(MA), clearly visible at first as a tiny point close to the cell membranes, began to increase in size with time. After having taken on increazing size, most of the larger MA became situated perpendicular or parallel to cell membranes, but near the cell center in the case of a few oocytes. The diameter of the aster of isolated MA at the metaphase of HG-treated oocytes was two or three times longer than that in the control experiment. Depending on its particular location in oocytes during maturation, the larger MA induced three diffrent events during meiotic division: giant polar body formation, unilateral furrowing in most oocytes, and equal cell division in a few oocytes. The frequency of these events depended on the time of transfer of oocytes to HG-SW during maturation.Based on these findings, we attempted to present an explanation for the events induced by HG. When maturing oocytes were transferred to HG-SW, HG diffused gradually into them, stabilizing the migrating MA in its particular position during maturation, and causing its size to increase through reassembly of microtubules around it. The larger MA may cause the meiotic division of oocytes to change, leading to the subsequent inducement of the above three events, depending on the particular location in the oocytes during maturation.