Researchs for Behavior Control of Insect Pests based on Host-selection of Insects.

研究了瓜实蝇Daccus cucubitae Cog.的空间构象与生物活性的相互关系，的果实中发现的。众所周知，生物活性物质的生物活性的表现取决于与生物体受体结构相关的多种因素的空间构象。在此基础上，我们对Teucrin Pl的两种空间异构体进行了研究，该萜类化合物在B环和E环上均具有船形构象。LDA/THF-HMPA处理后E环的船形构象变为椅形构象。NOE实验和X射线分析证实了这一点。在室温CHCl_3溶液中，经时相关圆二色性谱表明该异构体的稳定性，14天内60%的异构体转变为Teucrin P1。我们将继续研究由B环构象变化引起的生物活性的变化.发现亚麻酰甘油是一种具有良好引诱作用的物质。在田间未表现出引诱活性。为进一步解决这一问题，并从果实中探索新的引诱剂，我们进行了进一步的研究。从高极性组分中分离出对羟基苯甲酸甲酯，作为雄性果蝇的引诱剂。此外，从极性最强的组分中分离出对果蝇雌雄的引诱物。根据11 C和2D NMR谱推断该化合物为1-3-（β<beta>-吡喃半乳糖基）-2，3-二-o-亚麻酰基-x-甘油<13>。从同一级分中分离出了雌性和雄性果蝇的新引诱剂，并在500 MHz和2D NMR光谱上显示为6-亚麻酰基<beta>-D-吡喃葡萄糖基（1，3 '）-<beta>-麦角固醇。目前，我们正在进行这些糖苷化合物的合成，并在合成完成后，用亚麻酰甘油衍生物进行了现场试验。

The researches were performed on a interrelationships between steric conformation and biological activity, and attractants for female and male fruit fly, Daccus cucubitae Cog., in the fruits of Momordica Charantia L.1. It is well known that appearance of biological activity fepend on a steric conformation with ogher factors of bioactive compounds in relation to the structure of the receptor of organism. On based this knowledge, we performed to investigate two steric conformational isomers of teucrin Pl.This terpene compound had a boat conformation on the B and E rings. However, the boat conformation of the E ring chaged to the chair conformer by treatment with LDA/THF-HMPA. The fact was confirmed with NOE experiment and X-ray analyis. Stability of the conformational ismoer was shown on time-corse CD spectra in CHCL3 at room temperature, and the 60% amounts of the isomer had been changed to teucrin Pl for 14 days. We will continue to investigate the change of the biological activity resulting from the conformational change of the B ring.2. We had been founded that linolenoyl glycerol, which showed excellent attractant rantia L. did not showed the attractant acitivty in the field. We performed further to resolve this problem and to inverstigate new attractant from the fruits. From the higher polarity fraction, methyl p-hydroxybenzoate was isolated as the attractnat fro male fruit fly. Furthermore, the attractnats for female and male of frit fly was isolated from the most polarity fraction. This compound was deduced ad 1-3-(<beta>-galactopyranosyl)-2,3-di-o-linolenoyl-x-glycerol on the^<13>C and 2D NMR spectra. From the same fraction, thr new attractant for the female and male fruit fly was isolated and was shown that 6-linolenoyl-<beta>-D-glucopyranosyl(1,3')-<beta>-clerosterol on the 500 MHz and 2D NMR spectra. Now we performe to synthesys these glycoside componds, and on their completion, we well test in the field with linolenoyl glycerol derivatives.