Regulation of rejection reaction by Anti-Ia Antibody

本研究探讨了抗Ia抗体等体液因子对同种异体免疫反应的影响。与预期相反，在用抗Ia单抗(ISCR3)预处理的6只犬胰腺中，只有2只存活超过23天和29天。经抗Ia单抗灌流24小时后，未见明显的益处。预处理组功能周期为17.5±6.9d，对照组为12.1±1.6d。而同时接受ISCR3 10 mg的受者，移植胰腺功能明显延长28.0±7.7天，最长达38天。提示作为移植物免疫反应产物的抗体，对受体的免疫反应起调节作用，就像内镜子一样。接下来，我们用B-B杂交瘤和T-T杂交瘤技术对长期接受同种异体心脏移植的大鼠脾细胞的体液因子进行了研究。这些杂交瘤的一些上清液具有免疫抑制作用，这些上清液的作用种类繁多，从弱到很强，对非特异性的。研究这些杂交瘤的更多细节将为同种异体反应带来一些特异性免疫调节的可能性。

In This study, rolls of humoral factors such as Anti Ia antibodies on allogenic immune responses were investigated.Against the expectation, only two out of six pretreated canine pancreata with anti Ia monoclonal antibody (ISCR3) functioned for longer than 23 and 29 days. No apparent beneficial effect was seen with anti Ia monoclonal antibody perfusion for 24 hours of the grafts. Functioning period of pretreated group was 17.5+-6.9 days compare with functioning period of control group, 12.1+-1.6 days. However, group of recipients which reeeived 10mg ISCR3.simultaneously-onpancreas transplantation apparently functioned longer 28.0+-7.7 days, the longest was 38 days. It is suggested that antibodies which were outcome of immuneresponses against the graft work as modifier on immune reactions of recipients like as internal mirrors. Next,we investogated humoral factors using the methods of B-Bhybridoma and T-T hybridoma technic with spleen cells from the rat recipients of long term accptance of allogenic heart grafts. Some of supernatants of these hybridoma have immunosuppressive action and the effects of these supernatant were in wide variety, weak to very strong and specific to non-specfic. Studiesson more detail of these hybridomas would bring some possibilities of specific immune regulation to allo-reaction.