Investigation of angiogenesis factor. - Basic research for a treatment of brain ischemic diseases including moyamoya disease. -

本研究的目的是从人脑肿瘤、脑血管畸形和/或炎症组织中鉴定一种血管生成因子，用于治疗包括烟雾病在内的脑缺血性疾病。作为第一步，我们对人脑肿瘤手术标本进行了研究，在45 KD处发现了一个异常点，但其功能不明。作为下一步骤，尽可能多地积累脑肿瘤培养液的上清液，同时分离在手术过程中不可避免地去除的人脑毛细血管的内皮细胞。将分离培养的细胞系污染后，用市售细胞系检测细胞增殖活性。作为最后一步，检查脑膜瘤和建立的人神经胶质瘤细胞A-172的上清液。结果：脑膜瘤组织匀浆中的单个峰与对照组相比，细胞增殖活性无明显增加，而胶质瘤培养液上清中的单个峰与对照组相比，细胞增殖活性明显增加。在这两种情况下，电泳显示聚峰。

The purpose of this investigatio was to identify an angiogenesis factor from human brain tumor, cerebrovascular malformation, and/or inflamatory tissue for the treatment of cerebral ischemic diseases including moyamoya disease. As a first step operative specimen of human brain tumor was investigaied and an abnormal spot was identified at 45KD, but of which function was not specified. As a next step the supernatant of the brain tumor cultured fluid was acumulated as much as possible and at the same time separation of the endothelial cell of the capillary blood vessels of the human brain inevitably removed during the operation was carrued out. This separated and cultured cellline was later contaminated and commercially availavle cell line was used to cheque the cell proliferative acrivity. As a last step meningioma and supernatant of established human glioma cell A-172 were examined. In each case a single peak was obrained using heparin affinity and mono-S colums.Results; A single peak obtained from the homogenate of meningioma did not show significant incerease in cell proliferative activity as compared with controls whereas that obtained from the supernatant of the glioma cultured fluid showed significant increase in ability to promote cell proliferation. In both case the electrophoresis demonstrated poly peaks.