A serological study of tumour specific membrane antigens of cultured ovarian cancer cell lines

应用免疫粘附（IA）试验研究了培养的人卵巢癌细胞系的肿瘤相关抗原的体液抗体。用Kubo和L-Kubo两种卵巢癌细胞系检测了12例卵巢癌患者的173份血清。2例患者血清经牛红细胞吸附排除异源膜抗原抗体后，对L-Kubo细胞呈IA阳性，4例患者血清经Kubo细胞自身淋巴母细胞吸附后，对L-Kubo细胞呈IA阳性，经富含OFA的组织（胎脑）吸附后，血清样品中无反应性残留。这些结果表明，乳腺癌患者的血清中含有OFA-Ⅰ的抗体，但没有检测到卵巢组织类型特异性抗原，如IA使用两种卵巢癌培养细胞系测试。

Humoral antibodies to tumour associated antigens of cultured human ovarian cancer cell lines were studied using the immune adherence (IA) test. One hendred seventy three sera from 12 ovarian cancer patients were tested ageinst two ovarian cancer cell lines ; Kubo and L-Kubo. Sera from 2 patients were IA positive to L-Kubo cell line and sera from 4 patientes to Kubo cell line after absorption with bovine erythrocytes to exclude antibody to heterologous membrane antigens and after further absorption with lymphoblastoid cells autologous to these cell lines.Aftr absorption with OFA rich tissue (fetal brain), no reactivity remained in the sera samples. These results indicate the sera of patients with breast cancer contain antibodies to OFA-I,but do not detect ovarian histologic type specific antigens as tested by IA using two ovarian cancer cultured cell lines.