Bioactive function of alkaline protease inhibitor in soy bean miso.

我们已经尝试过同位素碱性蛋白酶抑制剂（API），并研究了其生理化学特性和生物活性功能。在两种不同类型的大豆misos（Edo-miso和Saikyo-miso）中检测到了API活动，其中Rice Koji是处理大豆miso的原始材料。对AP的抑制活动在大米koji、Saikyo-Miso和Edo-Miso的命令下很强，当使用API的crude准备时测试。该API进一步净化了Sephadex G-50凝胶过滤和DEAE-Toyopearl 650 S离子替代色谱柱。纯净的API的分子重量约为20，000，PI为5.6。API活动在70 ° C加热30分钟时受到严重影响，即使在100 ° C加热20分钟时受到严重影响，76%的API活动被检测到，因此API在热稳定性方面很高。它显示了pH值在9左右，pH值在9和11之间是稳定的，抗菌素测试使用8种微生物物种进行了自动生物学，但API并不能抑制这些物种的增长。然而， API抑制了32至64级的嗜酸卵的清除，以及DNA合成中API的抑制功能的事实。从结果来看， API在Miso中可能有一些生物活性的功能来抵消人类身体的增长，这一点令人沮丧。

We tried to isolate alkaline protease inhibitor(API) and studied its physicochemical properties and bioactive function. The API activity was detected in two kinds of soy bean misos(Edo-miso and Saikyo-miso) and rice koji which is the raw material for processing soy bean miso. The inhibiting activity to AP was strong in the order of rice koji, Saikyo-miso and Edo-miso, when tested by using crude preparations of API. The API was further purified from rice koji by Sephadex G-50 gel filtration and DEAE-Toyopearl 650S ion exchenge column chromatography. The molecular weight of the purified API was about 20,000 and the pI was 5.6. The API activity was scarcely affected by heated at 70゜C for 30 min. Even heated at 100゜C for 20 min, 76% of the API activity was detected, so the API was high in heatstability. It showed the optimum pH at around 9 and was stable between pH9 and 11.Antimicrobial test was carried out by auto-biography using 8 species of microorganisms, but API did not suppress the growth of these species. But, API inhibits the cleavage of 32 to 64 stage fertilized egg of starfish(Asterina pectinifera) and the fact suggested the inhibitory function of API in DNA synthesis. From the result, API in miso may have some bioactive functions to human body such as suppressing the growth of eukaryotic organisms.