Cell proliferation kinetics of chronic liver disease.

在慢性肝病中，活检组织的组织病理学结果、汇管区纤维化、炎性细胞浸润和碎片状或局灶性肝细胞坏死是非常重要的。然而，它们并不总是解释疾病的阶段。肝损伤和纤维化的程度在病原体的强度和肝细胞的再生能力的平衡上不时地变化。本研究通过检测肝活检组织中S期细胞的数量，并与病理组织学检查结果进行比较，以判断预后。材料与方法：20例肝活检组织。慢性活动性肝炎（CAH）12例，肝硬化（HC）4例，慢性非活动性肝炎（CIH）2例，慢性酒精性损伤（CAI）1例，治愈性急性肝炎（AH）1例。肝组织在含3 mg/ml溴脱氧尿苷（BrdU）的Eagle EME培养基中孵育1小时，3个大气压，95% ...更多信息 O_2和5%CO_2。孵育后，将组织固定在冷乙醇中并包埋在石蜡中。使用抗BrdU的单克隆抗体（MA-7580，Beckton-Dickinson）通过ABC法进行免疫组织化学染色。结果与讨论：所有标本均可见S期肝细胞，标记指数（LI）在1.8-27.3%之间。肝硬化（22.4 <plus-minus>± 4.2%）、慢性活动性肝炎（16.9 ± <plus-minus>3.8%）和急性肝炎（27.3%）的LI增高，CIH（3.6 <plus-minus>± 2.6%）和CAI（2.3%）的LI降低。因此，活动性慢性肝病的肝细胞显示出快速再生。纤维化程度按无、轻度、中度、桥接纤维化及间隔形成分级，并与LI进行比较。将炎症和肝细胞坏死程度分为无、轻度、中度和重度。结果表明，LI与汇管区纤维化程度之间存在较好的相关性。LI与炎性细胞浸润、肝细胞坏死无显著性差异

In chronic liver disease, the histopathological findings of the biopsied tissues, fibrosis of the portal area, inflammatory cell infiltration and piecemeal or focal hepatic cell necrosis are very important. However, they do not always explain the stage of disease. The degree of hepatic injury and fibrosis are changing from time to time on the balance of strength of the causative agents and regenerative capacity of the hepatic cells. In current study, we tried to detect S-phase hepatic cells in the biopsy specimen and compared with histopathological findings to estimate the prognosis.Material and Method: 20 cases of biopsied liver tissues were used for present study. 12 cases were chronic active hepatitis (CAH), 4 were cirrhosis (HC), 2 were chronic inactive hepatitis (CIH), 1 was chronic alcoholic injury (CAI) and 1 was healed acute hepatitis (AH). The liver tissues were incubated a hour in Eagle EME medium containing 3mg/ml Bromodeoxyuridine (BrdU), with 3 atmospheric pressure of 95% … More O_2 and 5% CO_2. After incubation, the tissues were fixed in cold ethanol and embedded in paraffin. Immunohistochemical staining by ABC method was performed using a monoclonal antibody against BrdU (MA-7580, Beckton-Dickinson). The BrdU positive hepatic cells were observed and the positive ratio (labeling index:LI) was calculated.Result and discussion: S-phase hepatic cells were demonstrated in all of the specimens examined, and LI were varied from case to case(1.8-27.3%). The cases of HC (22.4<plus-minus>4.2%), CAH (16.9<plus-minus>3.8%) and AH (27.3%) showed higher LI, and the cases of CIH (3.6<plus-minus>2.6%) and CAI (2.3%) showed low LI. Therefore, the hepatic cells of active chronic liver disease were revealed to regenerate rapidly. The degree of fibrosis was grade as non, slight, moderate, bridging fibrosis and septal formation, and compared with LI. The degree of inflammation and hepatic cell necrosis were graded as non, slight, moderate and marked. As the result, relatively well correlation was observed between LI and degree of fibrosis of the portal area. On the other hand, no significant meanings were noted between LI and inflammatory cell infiltration and hepatic cell nec Less