Study on proliferation and Differentiation of pluripotent hemopoietic stem cells (CFU-S).

本研究共包含三个独立研究计画，各计画在本年度期间之进展如下：1。多能造血干细胞（CFU-S）与衰老衰老过程中造血微环境的变化特别集中在对脾集落形成的影响方面。我们研究了从Dexter液体培养中收获的CFU-Ss和从用老年或年轻骨髓重新填充的受体小鼠骨髓中收获的CFU-Ss。其中一项实验得出结论：老年小鼠骨髓细胞与IL-3共培养2天，形成脾集落，老年小鼠CFU-s对IL-3的反应性不低于对照青年小鼠。欲了解更多详情，请参阅1988年在京都举行的日本血液学会年会的摘要。Hirabayashi et al. 2. CFU-S亚群的异质性与放射敏感性这一方案再次得到了一系列三组实验的证实，并在大剂量电离和非电离辐射生物效应国际会议上提出了总结。1988年，中国杭州。3. CFU-S和淋巴祖细胞。为了研究该计划，与Shin-ichi Aizawa博士（RIKEN）合作开发了转基因小鼠，其中所有造血干细胞都具有已引入的标记。这将在1989年日本病理学会年会上发表。

Shree independent programs are consisted this study, and each progress during the term of current fiscal year of the grant is as follows: 1. Pluripotent hemopoietic stem cells (CFU-S) and aging. Changes in hemopoietic microenvironment during aging were specifically focussed in terms of the effect on spleen colony formation. We investigated both CFU-Ss that were harvested from Dexter's liquid culture and that from bone marrow of recipient mice been repopulated with an old or a young bone marrow. One of experiments that came to a conclusion showed the responsibility to IL-3, of the CFU-s from old mice was not leser than that of control young mice, when bone marow cells that were harvested from old or yond donor, were cultured with IL-3 for 2 days, and let the cells from the culture form spleen colony. For further details, refer an abstract of the annual meeting of Japanese Hematological Society in Kyoto, 1988, presented by Y. Hirabayashi et al. 2. Heterogeneity of subfractions of CFU-S in radiosensitivity. This program was confirmed again by a series of three set of experiments, and the summary was presented at the International Conference on Biological Effects of Large Dose of Ionizing and Non-ionizing Radiation. 1988 in Hongzhou, China. 3. CFU-S and lymphogenous progenitor cells. In order to study this program, transgenic mice have been developed by a collaboration with Dr. Shin-ichi Aizawa (RIKEN) in which all of the hematopoietic stem cells have a marker that has been introduced. This will be presented at the annual meeting of Japanese Society of Pathology, 1989.