肝細胞癌の発生様式ー単中心性か多中心性かーに関する研究

采用Southern blot杂交技术分析了4只土拨鼠20例多灶性肝细胞癌的克隆来源。根据多灶性肿瘤的形态分类，将土拨鼠分为两组：(1)3只土拨鼠有多灶性肿瘤，相距较远，大小相近，提示肿瘤起源于多克隆；(2)1只土拨鼠有10个小的多灶性肿瘤，周围有2个大的主要肿瘤，提示原肿瘤有肝内转移。前一组的结果表明，土拨鼠肝炎病毒（WHV） dna的整合数量因肿瘤而异，且每个条带的大小不相同。在后一组中，两个大肿瘤周围的10个小肿瘤中有8个具有相同的WHV DNA整合模式，其中一个肿瘤表现出额外的条带，并与其他肿瘤共享相同的条带，而一个小肿瘤具有与其他肿瘤不同的整合模式。由此得出结论，杂交模式显示的克隆差异并不一定意味着hcc独立于不同的克隆，因为遗传变化可能在转移后或转移时发生。

The clonal origins of 20 multi-focal hepatocellular carcinomas (HCCs) in 4 woodchucks were analyzed by the Southern blot hybridization technique. The woodchucks were divided into two groups according to the morphological classification of the multi-focal tumors: (1) Three woodchucks had multi-focal tumors, widely separated and similar in size, which suggested a multi-clonal origin of the tumors, (2) one woodchuck had 10 small multi-focal tumors, surrounding two large main tumors, which indicated intrahepatic metastasis from an original tumor. Results of the former group demonstrated that the number of integrated woodchuck hepatitis virus (WHV) DNAs was different from tumor to tumor, and none of the bands were the same in size. In the latter group, eight of the ten small tumors surrounding the two large tumors snowed the same pattern of WHV DNA integration, one of them demonstrated an additional band as well as sharing the same bands with the other tumors, and one small tumor had a different pattern of integration from those of the others. It was concluded that clone dissimilarity demonstrated by hybridization patterns dose not necessarily mean that HCCs originate independently from different clone, because genetic changes may occur after or at the time of metastasis.