Research of the Mechanism of Calcific Deposition in Human.

[材料与方法]在平均2.5岁的青年母牛胫骨关节片（深度30 mm）的软骨面上挖两条平行的沟槽（间距25 mm）至软骨下骨。一种是用焦磷酸钠晶体填充，另一种是用氯化钙填充，并将切片在室温下放置五天。在距焦磷酸钠填充槽9mm处的两个槽之间形成白色区。该白色区由1.苏木精-伊红染色，Von Kossa染色2。SEM 3.通过X射线衍射、红外光谱和拉曼分析进行晶体学研究4.通过Kurata和Maeda方法进行酶分析。[结果]1.从切线层到过渡层基质钙化。2.扫描电镜观察到铺路石状晶体。3.晶体学分析表明钙盐为Ca_3（po_4）_2或Ca_5（po_4）_3OH。4.无机焦磷酸酶主要分布于放射状带以下的基质和软骨细胞以及浅层的软骨细胞中。用氟化钠处理的软骨实验既没有发现白色区，也没有发现无机焦磷酸酶活性的证据。[要点]软骨中的磷酸钙是由焦磷酸钠和氯化钙形成的。事实表明，关节软骨充当凝胶介质，其中化学物质能够扩散，晶体可以沉淀。在无机焦磷酸酶不存在的条件下，焦磷酸钙晶体可沉淀。

[ Material and Method ]Two parallel gutters (interval : 25mm) were digged to the subchondral bone from the cartilage surface of tibial articular slice (depth : 30mm) of young cows (average 2.5 years old). One was filled with sodium pyrophosphate crystal and the other was filled with calcium chloride and keep the slice in room temparature for five days. White zone was formed between two gutters, 9mm apart from the gutter filled with sodium pyrophate. This white zone was analyzed by 1. Hematoxylin-Eosin stain, Von Kossa stain 2. SEM 3. Crystallographic studies by X-ray diffraction, infrated spectroscopy and Raman analysis 4. Enzymatic analysis by Kurata and Maeda method.[ Results ]1. The matrix from tangential to transitional layer was calcified. 2. Pavementlike crystals were detected by SEM. 3. Calcium salts were Ca_3(po_4)_2 or Ca_5(po_4)_3OH by crystallographic analyses. 4. Inorganic pyrophosphatase was detected in the matrix and cartilage cells below radial zone and in cartilage cells of superficial layer. Some experiments on the cartilage treated by sodium fluoride revealed neither white zone nor the evidence of inorganic pyrophosphatase activites.[ Points ]Calcium phosphate was formed in cartilage from sodium pyrophosphate and calcium chloride. The fact showed that articular cartilage acted as a gel media in which chemicals are able to diffuse and crystals can precipitate. Calcium pyrophosphate cyrstals may be precipitated in condition that inorganic pyrophosphatase is absent.