Electrochemical Determination of Hydrogen-Peroxide at Ultra-Microelectrodes

体内定量测定需要分析物的空间分辨率，因为样品定位在活组织中。分析的另一个要求是不使用校准曲线的绝对测定，因为很少有可能用体外实验模拟体内实验条件。一种超微电极技术可以满足这些条件，特别是在直径8-10mum的碳纤维电极上。碳纤维电极的理论研究是在圆柱形模型的基础上进行的。它包括循环伏安法中的电极动力学。通过简单的氧化还原体系和过氧化氢的氧化实验验证了该方法的有效性。当溶液中含有微量杂质时，杂质会与过氧化氢发生催化反应。以邻菲罗啉亚铜配合物为例，根据不同的条件分别处于溶液、吸附和胶束状态。将过氧化氢的测定推广到自来水中臭氧的选择性分析。这项技术使我们能够从氯离子中分离臭氧。

Invivo quantitative determination requires spatial resolution in analyte because samples are localized in living tissues. The other requirement of the analysis is absolute determination without using a calikration curve because it is rarely possible to mimic invivo experimental conditions with invitro experiments. A ultro-microelectrode technique may fulfill these conditions, especially at carbon Fiber electrodes 8-10mum in diameter. Theoretical approaches on carbon Fiber electrodes have been made on a basis of a cylindrical model. It includes electrode kinetics in cyclic voltammetry. It has been verified experimentally by a simple redox system and oxidation of hydrogen peroxide. When solution involves a trace amount of imparities impurities cause catalytic reaction with hydrogen peroxide. As an example of the catalytic current, phenanthroline cuprous complex was selected, which was in th e solution, in absorption or in micellization depending on conditions. Determination of hydrogen peroxide was extended to selective analysis of ozone in tap water. This technique allows us to resolute ozone from chtorid ions.