Morphology and differentiation of human retinoblastoma

视网膜母细胞瘤(retinoblastoma，RB)是儿童最常见的眼内肿瘤，其组织发生机制最近被认为是由于一种原始的视网膜细胞，该细胞能够将Y79细胞系分化为多种细胞类型。通过对培养基组分的调整，以二丁基环丙基AMP为分化剂。电子显微镜、免疫组织化学检测谷氨酸脱羧酶(GAD)和细胞外基质(ECM)，如纤维连接蛋白和间质胶原(IV)。经处理的细胞表现出支持向光感受器分化的形态特征，增强了GAD活性，并表达了糖结合蛋白。研究发现，经处理的细胞以特定的模式合成细胞外基质。未见间质胶原合成。这些结果证实了人类RB起源于原始神经外胚层多潜能细胞的概念，并为RB的诱导分化治疗提供了可能性。

The histogenesis of retinoblastoma(Rb), the most common introocular tumor of childhood, has been recently attributed to a primitive retinal cell which is capable of differentiation of Y79 cell line into several cell types was investigated. By manipulating the culture medium constitutes and using differentiating agent as dibutyryl cysclic AMP. Electronmicroscopy, immunohistochemistry for detection of glutamate decarboxilase(GAD) and for extracellular matrix(ECM) such as fibronectin and stromal collagen(IV). Treated cells exhibited morphologic characteristics supportive of differentiation toward photoreceptors, increased GAD reactivity, and glycoconjugate expression. It was found that treated cells synthesize ECM in characteristic patterns. No stromal collagen synthesis was observed. These result confirm the concept that human Rb originates from a primitive neuroectodermal multipotential cell and contributes to the possibility of induction of differentiation therapy for Rb.