Research on the Control Mechanism of the Secretion of Ovarian Steroid Hormones

1)黄体酮可抑制大鼠垂体前叶促性腺激素的分泌。抗黄体酮药物RU486抑制了这种抑制。我们在体内和体外都证实了这一点。去索孕酮（DG）本身没有作用，而3酮-DG （DG的代谢物）降低促性腺激素的水平。2)研究合成类固醇对卵巢酶的影响。综上所述，孕酮抑制3 - β - hsd，酮-DG抑制3 -酮-DG，达那唑和DG抑制17,20裂解酶。本实验表明，DG直接抑制卵巢类固醇的合成。3)妊娠大鼠卵巢中存在EGF受体。我们将黄体细胞与EGF共培养2天，EGF抵消了促性腺激素的刺激作用，但对p4、20 α - oh - p4和cAMP的基础水平没有影响。4)妊娠大鼠卵巢中存在胰岛素和igf受体。我们用胰岛素或igf培养黄体细胞。当IBMX与这些肽一起使用时，它们增加了P_4, 20 α - oh -P_4和cAMP的水平。在没有IBMX的情况下，这些肽几乎没有作用，因为它们刺激cAMP磷酸二酯酶的活性。5) RU486对体外培养的黄体细胞有溶黄体作用，但对垂体去皮PMS-hCG处理大鼠血清P_4水平影响较弱。因此，在体内观察到的RU486的强溶血作用主要是通过垂体发挥的。

1) The gonadotropin secretion from the rat anterior pituitary was suppressed by progestin. RU486 (anti progestin drug) inhibited this suppression. We confirmed this both in vivo and in vitro. Desogestrel (DG) had no effect by itself, but 3 keto-DG (the metabolite of DG) decre ased the gonadotropin rease.2) We investigated the effect of synthetic steroids on ovarian enzymes. In conclusion, 3beta-HSD was inhibited by gestrinone, 3 keto-DG and 17,20 lyase was inhibited by danazol and DG. This experiment indicated that DG inhibited the synthesis of ovarian steroids directy.3) There is an EGF receptor in the ovary of pregnant rat. We cocultured luteal cells with EGF for two days and EGF counteracted the tropic effect of gonadotropins without any effect on basal levels of P_4, 20alpha-OH-P_4 and cAMP.4) There are receptors of insulin and IGFs in the ovary of pregnant rats. We cultured luteal cells with insulin or IGFs. When IBMX was used with these peptides, they increased the levels of P_4, 20alpha-OH-P_4 and cAMP. Without IBMX, these peptides had little effect because they stimu lated the activity of cAMP phosphodiesterase.5) RU486 had luteolytic effect on cultured luteal cells, but it had a weak effect on serum P_4 level of the hypophysectomized PMS-hCG treated rat. Therefore the strong luteolytic effect of RU486 observed in vivo was exerted mainly through the pituitary.