Atrial Natriuretic Peptide (ANP) in Childhood

(1)为探讨心钠素（ANP）在先天性心脏病（先心病）中的作用，测定了29例左向右分流先心病患者血浆ANP和25例右心耳手术患者血浆ANP。肺动脉ANP水平与平均肺动脉压呈正相关。心力衰竭合并右心房压力或容量超负荷者右心房心钠素水平升高。这些结果表明，心房中ANP的产生增加，作为心脏疾病中ANP持续释放到循环中的代偿性或继发性反应。(2)为了解心血管疾病患者心钠素的分子形式，我们采用反相高效液相色谱法（HPLC）结合放射免疫分析法（RIA）测定了心血管疾病患者右心房心钠素的含量。在20岁以下的患者中，主要成分是γ-ANP。心房组织中ANP含量和分子形式的差异反映了心血管疾病患者的病理状态，以及患者的年龄。(3)测定野百合碱诱导的肺动脉高压大鼠血浆和心房心钠素（ANP）水平。PH大鼠血浆ANP水平升高，组织ANP水平低于对照组。因此，肺动脉高压的发展导致右心房ANP释放增加。

(1) To investigate the ANP levels in congenital heart disease, we measured plasma ANP in 29 patients, with left-to-right shunt, and right auricle ANP in 25 patients who underwent operation. The levels of ANP in the pulmonary artery correlated with mean pulmonary arterial pressure. The right auricle ANP levels was high in patients with heart failure combined with pressure or volume overload of the right atrium. These results suggest that the production of ANP in the atrium increases as a compensatory or secondary response to persistent release of ANP into the circulation in cardiac disease.(2) To determine the molecular forms of ANP in patients with cardiovascular disease, we analyzed the ANP content in the right atrium using reverse phase HPLC with RIA. In patients younger than 20 yr old, the major component was gamma-ANP. The differences in the content and molecular forms of ANP in atrial tissue reflect the pathological state in patients with cardiovascular diseases, as well as the patient's age.(3) ANP level was measured in plasma and atrium of monocrotaline induced pulmonary, hypertensive rats. Plasma ANP levels were elevated in PH rats, and tissue levels of ANP were lower than those in controls. Thus, development of pulmonary hypertension led to an increase in release of ANP from the right atrium.