Transportation of structural constituents of the plant cell nucleus-Their ultrastructural localization during the cell cycle-

1.为了研究百合雄性生殖细胞质和核中类核仁的起源，应用核糖核蛋白（RNP）优先染色技术，在电子显微镜下对百合雄性生殖细胞中类核仁进行了详细的观察。在前中期完全分散在细胞质中的核仁衍生物质（核仁物质）在后期首先形成微小的无定形物质。末期早期，它们在细胞质中融合形成大的类核仁。巧合的是，部分核仁物质开始积聚在染色体表面并形成核核仁。2.核仁衍生结构（细胞质和核的类核仁、染色质上的核仁物质）RNA含量的测定（1）RNase-gold标记：在蚕豆根尖分生组织细胞中，所有的核仁衍生结构均未被RNase-gold标记。另一方面，在百合细胞中， ...更多信息 细胞质类核仁的标记率约为细胞核类核仁的50%。(2)rDNA探针原位杂交。- 百合细胞质类核仁被rDNA探针标记，表明其含有rDNA转录产物.间接免疫标记技术检测DNA。- 利用抗DNA单克隆抗体免疫标记技术，在蚕豆细胞中未发现所有的核仁衍生结构.秋水仙素和细胞松弛素B对核仁物质行为的影响。- 当维西蚕豆细胞暴露于秋水仙素时，分散的核仁物质形成大量的细胞质核仁，甚至在中期也在染色体表面积累。细胞松弛素B是一种F-肌动蛋白的抑制剂，在末期早期引起细胞质类核仁的显著增加。这表明细胞色素在核仁物质的运输中起一定作用.用银浸渍技术对连续切片进行了研究，结果表明，被运输到子核中的核物质进入了核线体。少

1. To investigate origin of the cytoplasmic and nuclear nucleoloids, detail examinations have been carried out under electron microscopy in Lilium male reproductive cells by applying ribonucleoprotein (RNP) preferential staining technique. The nucleolus-derived material (nucleolar material) completely dispersed in the cytoplasm during prophase-metaphase was found to first form minute amorphous masses in late anaphase. Then they fused each other to form large nucleoloids in the cytoplasm in early telophase. Coincidentally, part of nucleolar material began to accumulate on the surface of chromosomes and form nuclear nucleoloids.2. Estimation of RNA content of the nucleolar-derived structures (cytoplasmic and nuclear nucleoloids, nucleolar material on chromosoms)(1) RNase-gold labeling - In root-tip meristematic cells of Vicia faba, all the nucleolus-derived structures were not significantly labeled with the RNase-gold complex. In Lilium cells, on the other hand, the nuclear nucleoloids w … More ere moolerately labeled and the the labeling on the cytoplasmic nucleoloids was about 50% of that over the nuclear nucleoloids.(2) In situ hybridization using rDNA probe. - Lilium cytoplasmic nucleoloids were heavily labeled with rDNA probe, suggesting that they contain rDNA transcripts.3. Estimation of DNA by indirect immunolabeling technique. - No DNA molecule has been discerned for all the nucleolar-derived structures in Vicia faba cells by the immunolabeling technique using Anti-DNA monoclonal antibody.4. Effect of colchicine and cytochalacin B on behavior of the nucleolar material. - When cells of Vici faba were exposed to colchicine, the dispersed nucleolar material formed numerous cytoplasmic nucleoloids and accumulated on the chromosome surface even in metaphase. Cytochalacin B, an inhibitor of F-actin, caused remarkable increase of the cytoplasmic nucleoloids in early telophase. These suggest that cytoskelton plays a role to transport the nucleolar material.5. Silver impregnation technique applied to serail ultrathin sections has suggested that the nucleclar material transported into the daughter nuclei goes into the nucleolonema. Less