Experimental Research for Mechanism of Brain Abnormalities Induced by Intrauterine Infection with Cytomegalovirus.

用小鼠巨细胞病毒（MCMV）在妊娠第15天脑室内注射感染小鼠胎儿大脑。在第一位。我们对孩子出生后的大脑进行了神经病理学分析。我们使用神经元特异性烯醇酶抗体和MCMV早期抗原抗体进行免疫组化双染色，发现部分神经元易受MCMV感染，特别是在大脑皮层和海马。在出生后3至4周，约20%的mcmv感染小鼠在皮质和白质中观察到囊性脑病变。由于在囊性病变周围观察到血管周围的变化，我们使用人因子VIII抗体和抗mcmv抗体进行免疫组化双重染色。我们发现一些脑血管内皮细胞被MCMV感染。因此，囊性病变可能是由MCMV感染血管后的缺血后果形成的。

Brains of mouse fetus were infected with murine cytomegalovirus (MCMV) by intraventricular injection with MCMV on day 15 of gestation. At first. We have done neuropathological analysis of the brains of the offspring after birth. We found that some of the neurons were susceptible to MCMV infection, especially in the cerebral cortex and the hippocampus by immunohistochemical double staining using antibodies to neuron-specific enolase and antibodies to the immdiate-early antigen of MCMV. Cystic brain lesions in the cortex and the white matter were observed in about 20% of the MCMV-infected mouse 3 to 4 weeks after birth. As perivascular caffings were observed around the cystic lesions, we performed the immunohistochemical double staining using antibodies to human factor VIII and the anti-MCMV antibodies. We found that some of the endothelial cells of the brain blood vessels were infected with MCMV. Therefore, it is possible that cystic lesions may be formed by ischemic consequences after infection of the vessels with MCMV.