Influence of Fibroblasts on Chemosensitivity for Lung Cancer and Clinical Applications

为研究成纤维细胞对肺癌细胞的影响，采用体外MTT比色法测定了成纤维细胞对抗癌药物的敏感性，并对裸鼠移植肿瘤的体内发展进行了研究，探讨了成纤维细胞对肺癌细胞的临床应用前景。发现灵敏度是异质性的。将肺癌细胞与成纤维细胞混合培养（1:1），培养3天，检测对CDDP的敏感性。在18个培养中有11个培养中观察到相互作用，但在混合培养的同时添加CDDP时，这些培养都没有相互作用。因此，由成纤维细胞组成的条件培养基中的另一个因素似乎影响培养中的肺癌细胞，其作用被热处理破坏，但不受辐射影响。在体内研究中，移植到裸鼠体内的肺癌细胞中加入成纤维细胞更容易发生肿瘤发育，其生长也更大。临床上，涉及成纤维细胞促进肺癌细胞增殖的病例，往往在较早的阶段就表现出广泛的淋巴结转移。因此，某些类型的成纤维细胞似乎是影响肺癌细胞增殖的因素，并影响对抗癌药物的敏感性。作为一项体外试验，成纤维细胞条件培养基对肺癌细胞的影响（Luci 10）可能适用于预测肺癌个体患者的预后。

To examine the influence of fibroblasts on lung cancer cells, in vitro MTT colorimetric assay was conducted on their sensitivity toward anticancer agents as well as an in vivo study on the development of tumors transplanted in nude mice and possible clinical applications were investigated. Sensitivity was found to be heterogenous. Lung cancer cells and fibroblasts were combined in a culture ( 1 : 1 ), incubated for 3 days, and examined for sensitivity to CDDP. A mutual effect was observed in 11 out of 18 cultures, but not in any of these cultures when CDDP was added at the same time the culture was mixed. Another factor in the conditioned medium by fibroblasts thus appered to influence lung cancer cells in culture and whose action is destroyed by heat processing, but not affected by radiation. In the in vivo study, tumor development occurred more easily when fibroblasts were added to the lung cancer cells transplanted in to the nude mouse, and their growth was also greater. Clinically speaking, cases involving fibroblasts which promote proliferation of the lung cancer cells often show widespread metastasis to the lymph nodes at a comparatively early stage. Some types of fibroblasts thus appear to be factors influencing the proliferation of lung cancer cells as well as affecting sensitivity to anticancer agents. As an in vitro test, the influence of the conditioned medium by fibroblasts on lung cancer cells (Luci 10) may be applicable for predicting the prognosis of individual patients with lung cancer.