Anticonvulsant Effect of Nitrous Oxide

Nitrous oxide has a potent anticonvulsant action， but there are severa conflicting reports and the mechanism of anticonvulsant effect of nitrous oxide remains unknown.The purpose of this research is to investigate further the pharmacology of anticonvulsant action of nitrous oxide using various experimental models of induced convulsions。被雇用的以下卷积模型： 1）最大电击（MES） 2）药物诱导的卷积： a） Iidocaine（局部病理学）， b）五戊四唑和双氯丁胺（GABA拮抗剂）和（c）4-氨基吡啶（潜力频道阻断器）.在老鼠MES的混乱中，地震的严重程度被两个指标评估：音调外纹延伸的持续时间（TEF ; in sec）， and motor seizure pattern （score）。TFE的控制值为11.9<加minus>1.1 （MEAN<加minus>SD）秒，氮氧化物的剂量不足-取决于8.7<加minus> 0.9秒、6.3<加minus> 0.9秒、5.8<加minus> 0.5秒，30%、50%和70%氮氧化物，适当地 ... More .在MES腐蚀中，70%的氮氧化物的影响接近相等于5毫克/千克的二氮唑仑剂量。P.，两种药物对药物诱导的挥发性有联合作用，对空气中伊多卡因、五戊四唑、双库林和4-氨基吡啶的压力的变化，通过70%的氮氧化物进行了测试。氮氧化物明显增加了利多卡因、五戊四氮唑和双库林的平均干扰剂量的阈值，但氮氧化物在4-氨基吡啶的干扰中没有出现的抗干扰效应。However，4-氨基吡啶中毒被认为是用苯唑安定治疗的（5毫克/千克）。p）我们和其他人报告说，氮氧化物的急性耐受性现在已经上升到120分钟，在目前的研究中，有MES和氮氧化物的管理，没有找到急性耐受性。本研究建议，激活脑抑制剂系统是氮氧化物抗氧化剂作用的基础。它还可以被定义为什么这是一个直接的（E.）q. GABA受体-通道）氮氧化物的作用，或不。脑单胺在硝基氧化物的反硝基伏安离子中的作用也应进行调查。Less（低）

Nitrous oxide has a potent anticonvulsant action, but there are severa conflicting reports and the mechanism of anticonvulsant effect of nitrous oxide remains unknown.The purpose of this research is to investigate further the pharmacology of anticonvulsant action of nitrous oxide using various experimental models of induced convulsions. The following convulsion models were employed : 1) the maximum electroshock (MES) 2) drug induced convulsion : a) Iidocaine (local anesthetics), b) pentylenetetrazol and bicuculine (GABA antagonist), and c) 4-aminopyridine (potassium channel blocker).In the rat MES convulsion, severity of seizure was assessed by two indices : duration of tonic forelimb extention (TEF ; in sec), and motor seizure pattern (score). The control value for TFE was 11.9<plus-minus>1.1 (MEAN<plus-minus>SD) sec, and nitrous oxide suppressed it doseーdependently to 8.7<plus-minus>0.9sec, 6.3<plus-minus>0.9sec, 5.8<plus-minus>0.5sec, for 30%, 50% and 70% nitrous oxide, respectively … More . The effect of 70% nitrous oxide on the MES convulsion was approximately equivalent to a dose of diazepam of 5mg/Kg i. p., and both drugs acts synergFor drug induced convulsion, the change of the mean convulsion threshold dose of Iidocaine, pentyrentetrazol, bicuculine and 4-aminopyridine in air, by 70% nitrous oxide was examined. Nitrous oxide significantly increased the mean convulsion threshold dose of lidocaine, pentylenetetrazol, and bicuculine, but no anticonvulsant effect of nitrous oxide was seen on 4-aminopyridine convulsion. However, 4-aminopyridine convulsion was attenuated with diazepam (5mg/Kg i. p)Although we and others have reported on the acute tolerance of nitrous oxide up to now, in the present study, with MES and nitrous oxide administration up to 120 min, acute tolerance was not found.This study suggests that activation of brain inhibitory systems is the base for the anticonvulsant action of nitrous oxide. However it remains to be defined whether this is a direct (e. q. GABA receptor-channel) action of nitrous oxide, or not. The role of brain monoamines in the anticonvulsant ac ion of nitrous oxide should also be investigated. Less