The Sensitivity Test for Human Urinary Tract Malignancies Making of use of the Chick Embryo Method using Chorioallantoic Membrane

在单独化疗、单独热疗或两者结合对人泌尿道恶性肿瘤的敏感性试验中，使用使用绒毛尿囊膜的鸡胚方法，测量肿瘤块的重量被认为是评价抗肿瘤效果的有效方法。然而，在大部分肿瘤坏死的情况下，这种评估方法不能提供可靠的结果。目前，采用MTT法，定量结果较为准确。作为使用连续培养的细胞系的转移性肿瘤的敏感性试验，从受精的鸡胚的转移器官中提取DNA，进行特异性DNA扩增和免疫电泳，并测量DNA含量。通过该方法，表明可以定量评价各种治疗方法的抗肿瘤效果。

In sensitivity tests of chemotherapy alone, hyperthermia alone, or a combination of the two against human urinary tract malignancies making use of the chick embryo method using chorioallantoic membrane, measurement of the weight of the tumor mass was considered to be an effective method of evaluating the antitumor effect. However, in cases in whicha a large part of the tumor is necrotic, this evaluation method did not provide reliable results. At present, using the the MTT method, more accurate quantitative results have been obtained. As a sensitivity test for metastatic tumors using continuously cultured cell lines, DNA is extracted from the metastatic organs of a fertilized chick embryo, specific DNA amplification and immuno-electro-phoresis are performed, and the DNA content is measured. By this method, it was shown that is possible to quantitatively evaluate the anti-tumor effect of various treatment methods.