Analysis of Carbohydrate Antigen Involving in sterility

众所周知，血清中含有精子固定抗体的妇女很少能怀孕，尽管常规医学检查其生殖器官没有异常。一种单克隆抗体（MAb）2 H12。用人绒毛膜癌细胞系JEG-3免疫BALB/C小鼠，HAb 2 H12具有较强的精子固定活性，并与硫脂和磷脂反应。单克隆抗体2 RI 2的精子固定活性明显被硫苷脂或硫代糖脂吸收，但其他几种用人精子或精浆免疫制备的精子固定单克隆抗体不能被相同的硫代糖脂吸收。不明原因不孕妇女血清中的精子固定抗体也能被硫苷脂或磷脂吸收。MAb 2 H12缀合的免疫珠粒（MAb 2 H12-IMB）结合至活动精子。MAb 2 H12-IMBs与精子的这种结合被MAb 2 H12或含有精子固定抗体的妇女血清竞争性抑制，但不被正常妇女血清或几种其它精子固定MAb与人精子的结合抑制。这些结果表明，妇女血清中的精子固定抗体是针对精子膜上磷脂的3-O-磺基半乳糖残基的抗体。

It is well known that very few women who possess sperm immobilizing antibodies in their sera can conceive, though there are no abnormalities in their reproductive organs by routine medical examinations. A monoclonal antibody (MAb) 2Hl2. was produced by immunizing BALB/C mouse with human choriocarcinoma cell line JEG-3. The HAb 2Hl2 showed strong sperm immobilizing activities and reacted to sulfatide and seminolipids. The sperm immobilizing activities of MAb 2RI2 were clearly absorbed with sulfatide or seminolipid but several other sperm immobilizing MAbs that were made by immunization with human speria or seminal plasma could not be absorbed with the same sulfoglycolipids. The sperm immobilizing antibodies in the sera of sterile women with unknown cause were also absorbed well with sulfatide or seminolipid. The MAb 2HI2 conjugated immunobeads (MAb 2Hl2-IMBs) bound to motile sperm. This binding of MAb 2Hl2-IMBs to sperm was competitively inhibited either by MAb 2Hl2 or women's sera containing sperm immobilizing antibodies, but was not by normal women's sera or several other sperm immobilizing MAbs to human sperm. These results suggest that the sperm immobilizing antibody in women's sera is antibody against 3-0-sulfogalactose residue of seminolipid on the sperm membrane.