Production of anti-idiotype antibody against human monoclonal sperm immobilization antibody

制备了人单克隆精子固定抗体（H6-3C4）的小鼠抗独特型抗体，利用动物和人精子固定抗原内显像分析避孕疫苗的可能性。用人γ球蛋白吸附H6-3C4免疫小鼠血清，纯化的抗血清（AB2）与H6-3C4反应。AB2能中和H6-3C4和En46-A4对人精子的固定作用。AS En46-A4是通过重组DNA技术从H6-3C4 （IgM）中分离出的一类SI-ab （IgGl）抗体，两种抗体使用相同的V基因作为免疫球蛋白。然后它们对不同种类的免疫球蛋白具有相同的抗原结合特异性（paratope）。经AB2免疫的大鼠和家兔血清具有明显的人精子固定活性。这些数据表明，AB2含有大鼠和家兔精子固定抗原的内部图像，可以替代动物和人的纯化精子抗原作为避孕疫苗的候选物。然后，我们制备了抗Mab H6-3C4的小鼠单克隆抗独特型抗体（Mono AB2），以便应用于人。4个Mono AB2s对H6-3C4具有特异性，1个Mono AB2对H6-3C4的精子固定化活性具有中和作用。大鼠抗单抗AB2血清对人精子无固定作用。现在我们正在制作另一种小鼠单声道AB2。

Anti-idiotipic antibodies in mice against human monoclonal sperm immobilization antibody (H6-3C4) were produced to analyze the possibility for contraceptive vaccine by using anti-idiotype antibodies for internal image of antigen of sperm immobilization in animals and human. Sera from mice immunized with H6-3C4 were absorbed with human gamma globulin and purified antisera (AB2) were confirmed to react the H6-3C4. AB2 neutralized both sperm immobilizing activity of H6-3C4 and En46-A4 to human sperm. AS En46-A4 is class switched human SI-ab (IgGl) from H6-3C4 (IgM) by recombinant DNA technology, both antibodies were used same V gene for immunoglobulin. Then these have same antigen binding specificity (paratope) on the differrent class of immunoglobulin.Sera from rats and rabbit immunized with AB2 showed significant human sperm immobilization activity. These data suggested that AB2 contained internal image of sperm immobilizing antigen for rat and rabbit and could be suitable candidate for contraceptive vaccine instead of purified sperm antigen in animals and human.Then, we produced murine monoclonal anti-idiotype antibody against Mab H6-3C4 (Mono AB2) in order to apply for human. Four Mono AB2s were specific for H6-3C4 and one Mono AB2 had neutrizing activity of sperm immobilizing activity of H6-3C4.Rat antiserum against Mono AB2 had no sperm immobilizing activity for human sperm. Now we were making another murine Mono AB2.