Investigation of Dynamical Properties of Proteins by Hole-Burning Spectroscopy

1. Holes burned into visible absorption bands of myoglobin substituted with a Zn ion or twohydrogen ions. Observed phonon sideband profiles were almost identical for myoglobin solution andfreeze-dryed myoglobin.那是确认the phonon sideband profiles reflect not thevibration of the solvent but the vibration of the protein.2. In order to investigate thehole-filling process across the barriers between the initial and the final state of the hole-burningprocess,a heat cycle experiment was carried out.它包含了barrier height distributionobtained from the experiment on Zn-substituted myoglobin reflects the能量结构self and shows no markable peak structure while the distribution obtained from the h_2 -substituted myoglobin corresponds to the barrier between the tautomers of the central hydrogens inporphyrin and shows sharp peak structure.3. Phonon sideband profiles calculated on the basis ofnormal mode analysis of the internal motion of myoglobin using linear approximation forelectron-phonon interaction. The results compared with experimental results4.我们tried the hole-burning experiments on，以及他们在该地区above 20 wave numbers.4UV吸收bands of proteins which have no visible吸收bands. As a result，holes burned into the absorption bands of tyrosine，和phonon-sidebands被observed，但它有不同于obtain accurate profile of thephonon - sideband because of saturation effect

1. Holes were burned into visible absorption bands of myoglobin substituted with a Zn ion or two hydrogen ions. Observed phonon sideband profiles were almost identical for myoglobin solution and freeze-dryed myoglobin. Thus it is confirmed that the phonon sideband profiles reflect not the vibration of the solvent but the vibration of the protein.2. In order to investigate the hole-filling process across the barriers between the initial and the final state of the hole-burning process, a heat cycle experiment was carried out. It was concluded that the barrier height distribution obtained from the experiment on Zn-substituted myoglobin reflects the energy structure of protein itself and shows no remarkable peak structure while the distribution obtained from the H_2 - substituted myoglobin corresponds to the barrier between the tautomers of the central hydrogens in porphyrin and shows sharp peak structure.3. Phonon sideband profiles were calculated on the basis of a normal mode analysis of the internal motion of myoglobin using linear approximation for electron-phonon interaction. The results were compared with experimental results, and they agree well in the region above 20 wave numbers.4. We tried the hole-burning experiments on UV absorption bands of proteins which have no visible absorption bands. As a result, holes were burned into the absorption bands of tyrosine, and phonon-sidebands were observed. But it was difficult to obtain accurate profile of the phononーsideband because of saturation effect.