Three-dimensional molecular status of bone morphogenesis as revealed from molecular biological analyzes.

例如，脊椎动物腿的发育过程完全编码在由肢芽（腿原基）组成的遗传信息中。在骨形态发生过程中，遗传信息的表达受到精确的时空控制，程序化过程与发育过程中表观遗传表达的位置信息协同进行，本研究旨在揭示骨形态发生的三维分子状态，采用两种不同的实验体系，分别在发育组织水平和相应的骨细胞水平上进行。一个是软骨内骨化系统，如肢芽发育，另一个是膜内骨化系统，如颅面发育。我们的分子分析集中在：（1）具有位置信息的所谓形态原的实质性实体，（2）导致体轴建立或胚胎发育期间上皮间充质相互作用的同源框基因表达的分子控制，（3）生长/分化因子的动态分布模式，特别是在肢芽内以三维方式释放，其定向细胞-细胞分化相互作用，最后，（4）骨形态发生神经元联合的分子机制。脊椎动物胚胎发育过程中骨形态发生的三维分子状态。

The developing process of vertebrate legs, for example, is wholly encoded in the genetic information comprised by the limb buds, the leg primordia. During the bone morphogenesis, the genetic information is expressed under precise tempo-spatial control, thus the programd process proceeds in cooperation with positional information epigenetically expressed in the course of development.The present research project, aiming at revealing three-dimensional molecular status of bone morphogenesis, has sofar been promoted either at developing tissue level or the relevant bone cell level with two different experimental systems. The one is enchondral ossification system such as the limb bud development, and the other intramembraneous ossification system such as craniofacial development.Our molecular analyzes have focused on : (1)substantial entity of so-called morphogen, bearing positional information, (2)molecular control of homeobox gene expression leading to establishment of body axs, or of epitheliomesenchymal interaction during embryonic development, (3)dynamic distribution pattern of growth/differentiation factors, specifically released in three-dimensional fashion inside the limb buds, which orientates the cell-cell differentiating interaction, and finally, (4)molecular mechanism of neuron association of bone morphogenesis.Our results sofar obtained primarily elucidate basic three-dimensional molecular status of bone morphogenesis during development of vertebrate embryos.