Molecular phylogeny of Yeasts and Yeast-like Fungi Based on Partial Sequences of 18S and 26S rRNAs

在过去3年的研究期间，我们发表了14篇原创论文。1)紫球藻属的特征在于细胞中存在木糖并形成淀粉样化合物，并被认为是隐球菌属的同义词。18 S和26 SrRNA部分序列测定结果表明，该属是一个独立的属。2)Aexula货车der Walt，Smith et Yamada属的无性型子囊菌酵母具有嘌呤碱基同化的特性，嘌呤碱基是唯一的碳源，也是子囊菌的有性型酵母属Stephanocyte共有的抗原。本实验结果表明，上述两个属的18 S和26 SrRNA的部分碱基序列非常接近。3)对5种具有Q10的红孢子虫进行了18 S和26 S rRNA部分序列的检测。在所调查的5个种中，仅有R.在18 SrRNA部分序列中，dacryoacetate有11个碱基的差异。结果表明，该种应归入独立的属。4)动形子囊酵母。Holleya Yamada属的醌系统为Q-9（Q-8）。18 SrRNA序列分析表明，H. sinecauda和Nematospora coryli（Q-5或Q-6），表明Holleya属应独立于Nematospora属。

During the last 3 years of the research period, we have published 14 original papers. 1) The genus Phaffia is characterized by the presence of xylose in the cells and the formation of starch-like compounds, and recognized as a synonym of the genus Cryptococcus. The determination of 18S and 26S rRNA partial sequences showed that the genus Phaffia exsist as a proper genus. 2) The anamorphic, ascomycetous yeast genus Aexula van der Walt, Smith et Yamada has the properties of the assimilation of purine bases as a sole source of carbon and of the antigens common to the ascomycetous, teleomorphic yeast genus Stephanoascus. The present experiment indicated that the above-mentioned two genera had partial base sequences in 18S and 26S rRNAs very close to each other. 3) The five Q10-equipped Rhodosporidium species were examined for partial sequences of 18S and 26S rRNA. Of the five species examined, the only species, R. dacryoidum had 11 base differences in the 18S rRNA partial sequence. The results obtained indicated that the species should be classified in its own separate genus. 4) The teleomorphic ascomycetous yeast. genus Holleya Yamada is characterized by the quinone system of Q-9 (Q-8). The sequence data obtained in 18S rRNA showed that the number of base differences was 7 between H. sinecauda and Nematospora coryli (Q-5 or Q-6), and indicated that the genus Holleya should be recognized as a genus separate from the genus Nematospora.