RESEARCH IN IMMUNOLOGICAL DIAGNOSIS AND VACCINATION THERAPY AGAINST BRAIN TUMORS USING ANTI-IDIOTYPIC ANTIBODY.

1.用识别人、大鼠和小鼠共同抗原的单克隆抗体（RS-11，Ab_1）免疫小鼠，制备大鼠抗独特型抗体（Ab_2）。用Ab_2免疫小鼠，可诱导小鼠产生抗抗独特型抗体（Ab_3），并使脾脏杀伤细胞活性增强。Ab_2免疫大鼠胶质瘤细胞后，移植瘤生长受到抑制。抑制性T淋巴细胞的活性增强了这种肿瘤生长抑制作用。2）使用识别人脑肿瘤相关抗原（TA 226）的人单克隆抗体（CLN-IgG）制备抗独特型抗体（Idio-33）。Idio-33为β型抗独特型抗体。在重建了人类免疫系统的scid小鼠中，通过免疫Idio-33的治疗发现了对人类胶质瘤生长的抑制作用。在scid小鼠血清中诱导出Ab_3。CLN-IgG治疗患者的外周淋巴细胞在Idio-33刺激下产生Ab_3。抗独特位在B淋巴细胞和辅助性T淋巴细胞的细胞表面表达。

1), Rat anti-idiotypic antibody (Ab_2) was prepared by the immunization of monoclonal antibody (RS-11, Ab_1) recognized of the common antigen in species of human, rat and mouse. The anti-anti-idiotypic antibody (Ab_3) and the increased activity of killer cell in spleen were induced by the immunization of Ab_2. When the rat glioma cells were transplanted following the immunization of Ab_2, tumor growth was inhibited compared to controls. This tumor growth inhibiting effect was exaggerated by the inhibition of the activity of suppressor T lymphocytes.2), Anti-idiotypic antibody (Idio-33) was prepared using the human monoclonal antibody (CLN-IgG) recognized of the human brain tumor associated antigen (TA226). Idio-33 was beta type of anti-idiotypic antibody. In the scid mice with the reconstruction of human immune system, the inhibitory effect of tumor growth of human gliomas were found by the treatment with the immunization of Idio-33. And Ab_3 was induced in the sera of scid mice. The peripheral lymphocytes in the patients treated with CLN-IgG produced the Ab_3 by the stimulation with Idio-33. Anti-idiotope was expressed in the cell surface of B lymphocytes and also helper T lymphocytes.