Development of Analytical Instruments and Establishment of Analytical Method for Volatile Drugs and Poisons in Minute Amounts of Biological Samples

目前的研究是为了开发分析仪器和建立能够准确和快速分析挥发性物质（如乙醇、氯仿和甲苯）的程序，即使是微量的生物标本。得到的结果如下：1。为了揭示铁磁合金重复使用的可能性，实验制作了特殊的铝胶囊，以防止铁磁合金分析和变质后的污染。在动物组织样品中乙醇、二乙醚、氯仿和甲苯的基础实验中，脉冲加热法的测量值与顶空法的测量值基本一致。结果表明，脉冲加热法可用于测定所有被检挥发物。样品标本分为5份，每份标本在5个不同的研究所同时检测：1)…More In乙醇浓度测量值相互比较；A研究所与B研究所之间（相关系数(r)=0.943）；A与C之间（r=0.928）；A与D之间（r=0.990）；A与E之间存在显著性正相关（r=0.956）。2)比较了二乙醚、氯仿和甲苯的浓度。A与C的相关系数为0.601，A与E的相关系数为0.858。这些系数变化很大。这个问题需要进一步调查。采用脉冲加热法和头部空间法测量了尸体尸体血液和尿液中的乙醇浓度。两种方法测得的值均有如下回归公式：Y=0.117+0.993 3x (Y为脉冲法测得的值，X为顶空法测得的值，r=0.996，样本量，n=85)，具有较强的正相关关系，表明脉冲加热法在定性和定量分析中均可准确、快速地分析微量样品中的乙醇。二乙醚、氯仿和甲苯的测量数据在不同的研究所之间差异很大。这需要进一步调查。脉冲加热对挥发物的快速定性筛选非常有用。少

The present research has been performed in order to develop analytical instruments and to establish the procedures, being capable of accurate and rapid analysis for volatile substances (e.g., ethanol, chloroform and toluene) even in minute amounts of biological specimens. The results obtained are as follows;1. In order to reveal the possibility of repeated usage of ferromagnetic alloys, special aluminum capsules were made experimentally to prevent contamination after analysis and deterioration of ferromagnetic alloys.2. In fundamental experiments with animal tissue samples for ethanol, diethylether, chloroform and toluene, the measured values by the pulse heating method almost correlated to those by the head space method, respectively. It was also revealed that the pulse heating method could be available for the determination of all of the volatiles examined.3. Sample specimens were divided into 5 portions, and the each specimen was examined simultaneously at 5 different institutes:1) … More In ethanol concentrations measured values were compared to each other; between institute A and B (correlation coefficient(r)=0.943); between A and C (r=0.928); between A and D (r=0.990); between A and E (r=0.956), indicating strong positive correlations.2)The concentrations of diethylether, chloroform and toluene were also compared. The correlation coefficient between A and C was 0.601 and between A and E, 0.858. These coefficients wide variations. The problem required a further investigation.4. Ethanol concentrations in the blood and urine obtained from autopsied cadavers were measured by the pulse Heating and the head space methods. The measured values by the two different methods showed the following regression formula : Y=0.117+0.993X (Y, the measured value by the pulse method; X, those by the head space method; r=0.996; sample size, n=85), indicating a strong positive correlationFrom these results, it has been revealed that pulse heating method was available for accurate and rapid analysis for ethanol in minute amounts of samples in both qualitative and quantitative analysis. The data obtained by measuring of diethylether, chloroform and toluene showed a wide variation among the different institutes. This required a further investigation. The pulse heating is, very useful for rapid qualitative screening of volatiles. Less