Bioleaching of silver sulfide

为了考察氧化亚铁硫杆菌在硫化银浸出中的应用，研究了银离子对氧化亚铁硫杆菌亚铁离子和单质硫氧化活性的影响。本研究中使用的11株氧化亚铁杆菌是从日本硫化矿和冶炼厂的水中分离出来的。银离子抑制铁离子氧化T. ferrooxidans的氧化活性。氧化速率随银浓度的增加而降低。细菌能氧化的最大银浓度。90%的亚铁离子（初始浓度4.0 × 10^<-2>mol/1）从0。LLPPM至0.28ppm。在铜浓度逐渐增加至13g/l的培养基中生长的耐铜菌株具有较高的耐银性。菌株在1 . Oppm的银浓度下氧化90%的亚铁离子。耐镍菌株在镍浓度逐渐增加至log/l的培养基中生长，其耐银性较差。银浓度为5ppm时，T. ferrooxidans的单质硫氧化活性受到抑制。在银浓度逐渐增加的培养基中生长的适应菌株在银浓度为5ppm时氧化单质硫。

To examine the application of Thiobacillus ferrooxidans in the leaching of silver sulfide, effect of silver ion on the ferrous ion and elemental sulfur oxidation activities of T. ferrooxidans was investigated.11 strains of T. ferrooxidans used in this study were isolated from the waters of sulfide mineral mines and a smelter in Japan.Silver ion inhibited ferrous ion oxidation activity of T. ferrooxidans. Oxidation rate decreased as silver concentration increased. Maximum silver concentration that bacteria could oxidize. 90% of ferrous ion(initial concentration 4.0x10^<-2>mol/1)at is from 0. llppm to 0.28ppm. A Copper tolerant strain that grew, -in the mediums with gradually increasing copper concentration up to-13g/l had higher silver tolerance. The strain oxidized 90% of ferrous ion at the silver concentration of l. Oppm. A nickel tolerant strain that grew in the medium with gradually increasing nickel concentration up to log/l had less silver tolerance.Elemental sulfur oxidation activity of T. ferrooxidans was inhibited at the silver concentration of 5ppm. An adapted strain that grew in the medium with gradually increasing silver concentration oxidized elemental sulfur at the silver concentration of 5ppm.