Solubilization of Biological Substance by Using Water-Soluble Polymers

1.原卟啉在水和聚(N-乙烯基吡咯烷酮)水溶液中的缔合行为：利用吸收光谱和凝胶层析法研究了不同pH条件下原卟啉IX的缔合行为。原卟啉IX在酸性水中以单体形式存在，在中性水中以聚集体形式存在，在碱性水中以二聚体形式存在。聚(N-乙烯基吡咯烷酮)(PVP)倾向于抑制原卟啉IX2的二聚化和聚集。叶绿素(铜-钠盐)在水和聚N-乙烯基吡咯烷酮溶液中的凝胶层析法：比较了叶绿素(铜-钠盐)在水和PVP水溶液中的凝胶色谱。吸收光谱中430 nm处的肩峰是由叶绿素单体(铜钠盐)引起的。PVP有抑制叶绿素(铜钠盐)聚集的趋势。水溶性聚合物与叶绿素a、β-胡萝卜素的相互作用：超声波处理对叶绿素a红峰的波长和半峰宽没有影响，凝胶层析显示，β-胡萝卜素的红移吸收带(&gt；495 nm)没有与其他较短波长的吸收带分离。氨对中性水中胆红素的增溶作用

1. Association Behavior of Protoporphyrin in Water and Aueous Poly(N-vinylpyrrolidone)Solutions :The association behavior of protoporphyrin IX was investigated at different pH by absorption spectroscopy and gel chromatography. Protoporphyrin IX existed as monomer in acidic water as aggregate in neutral water, and as dimer in basic water. Poly(N-vinylpyrr olidone)(PVP)tended to suppress dimerization and aggregation of protoporphyrin IX.2. Gel Chromatography of Chlorophyllin(Cu-Na salt)in Water and Aqueos Poly(N-vinylpyrrolidone)Solution :Gel chromatography of chlorophyllin(Cu-Na salt)in water was compared with that in aqueous PVPsolution. A shoulder at 430 nm in the absorption spectra was due to monomer of chlorophyllin(Cu-Na salt). PVP tended to suppress aggregation of chlorophyllin(Cu-Na salt).3. Interaction between Water-Soluble Polymer and Chlorophyll a, beta-Carotene :The treatment of ultrasonic wave had no effect on the wavelength and half band width of red peak of chlorophyll a. By gel chromatography, a red-shifted absorption band(>495 nm)of beta-carotene was not separated from the other bands of shorter wavelength.4. Solubilization of Bilirubin in Neutral Water by Using Ammonia :