Elucidation of Biosynthesis and Metabolism of Nicotinic Acid Related Compounds and Various Specific Physiological Functions of the compounds

1. 枸杞酸（Cinchomeronic Acid， CA）对萝卜幼苗的促生长作用(1)：在施用10mM时，萝卜茎长达到最大值，是对照的2.18倍。显微镜下观察萝卜茎表皮细胞的细胞长度比对照长数倍，细胞宽度约为对照的一半。吲哚乙酸对萝卜幼苗的生长有轻微的促进作用。(2)对大肠杆菌和其他微生物：在0.1 ~ 100mm的CA中，大肠杆菌B对各种微生物（乳酸菌、大肠杆菌、枯草芽孢杆菌、酵母菌）效果最好。在含有[3,4,7,8 -^<14>C] CA的培养基中，浮萍和大肠杆菌B提取物均未观察到除CA外的放射性峰。[^<14>C] CA与浮萍和大肠杆菌b的无细胞提取物孵育后，反应混合物中除CA外没有观察到放射性峰，因此CA可能不会在这些生物体中代谢。各种烟酸相关化合物对培养动物细胞的作用(1):3种动物细胞培养基中添加的化合物几乎都有抑制作用。(2)浮萍的诱导开花：未发现内在烟酸-、烟酰胺-和nad -含量与诱导开花的关系。甘氨酸在海洋无脊椎动物体内的生物合成目前提出的甘氨酸生成甘氨酸的途径还没有得到各种研究的支持。

1. Growth-prmoting Effect of Cinchomeronic Acid (CA)(1) On Radish Seeding : Maximum growth of the stem was observed at 10mM of CA and it was 2.18 times longer than that of control. Cell length was several times longer and cell width was about the half as compared to those of the control on microscopic obervation of the epidermal cells of radish stems. Indoleacetic acid slightly promoted the growth of radish seedings.(2) On Escherichia coli and Other Microorganisms : At 0.1-100mM of CA with E. coli B was most effective among various microorganisms (lactic acid bacteria, E. coli, Bacillus subtiis, yeast).2. Metabolism of Cinchomeronic Acid in Plants and Microorganisms No radioactive peak other than CA was observed in the extract from duckweed and E. coli B grown in the medium containing [3, 4, 7, 8-^<14>C] CA on radio HPLC analysis. Also no radioactive peak except CA was observed in the reaction mixture after incubating [^<14>C] CA with the cell-free extract from duckeweed and E. coli B. Hence, CA may not be metabolized in the these organisms.3. Function of Various Nicotinic acid-Related Compounds(1) On Cultured Animal cells : Almost all compounds added to the medium of 3 kinds of animal cells were inhibitory.(2) On Flowering Induction in Duckweed : Relationship between intrinsic nicotinic acid-, nicotinamide-, and NAD-contents and flowering induction was not found.4. Biosynthesis of Homarine in marine InvertebratesProposed pathway from glycine to homarine can not be supported with various investigations.