Expression of RB gene and EIA oncoprotein in virus-induced retinal tumor

为了阐明视网膜母细胞瘤发生的早期遗传变化，我们利用不同的DNA探针研究了人腺病毒12型诱导大鼠视网膜肿瘤的基因组表达。22只大鼠在出生后24小时内单次眼内接种浓缩病毒液，接种后118天内，22只动物中有1只在玻璃体内诱发肿瘤。采用southern blot杂交技术检测肿瘤中腺病毒相关癌蛋白基因ElA和人视网膜母细胞瘤易感基因H3-8的表达。原位杂交分析表明，接种后3小时内，腺病毒12型ElA基因在视网膜内颗粒层表达。之后，N-myc在转化细胞中的活性逐渐增加。这表明，腺病毒12型ElA片段与宿主基因组的整合以及N-myc扩增后基因的表达是该肿瘤进展所必需的。

To elucidate the early genetic change in tumorigensis of retinoblastoma, we investigated the genomic expression in retinal tumors induced by human adenovirus type 12 in rats, using various DNA probes. Twenty two rats received a single intraocular inoculation of concentrated virus fluid within 24 hours after birth, Intravitreous tumors were induced in one out of 22 animals ithin 118 days after the inoculation. The adenovirus related oncoprotein gene ElA and human retinoblastoma susceptibility gene H3-8 were detected in the tumors by southern blot hybridization. In sitt hybridization analysis demonstrated expression of adenovirus type 12 ElA gene in the inner granular layer of the retina within 3 hours after the inoculation. After then, N-myc gradually increased activity in transformed cells. It was suggested that integration of adenovirus type 12 ElA fragment with the host genome and expression of the gene following amplified N-myc were required for progression of this tumor.