Mechanism of stress ulcer - analysis from the viewpoint of neuro-endocrine-immune system.

急性食物限制被认为会引起人类的心身压力，并已被证明会在小鼠胃中产生应激性溃疡。近年来，越来越多的证据表明，应激激素、某些淋巴因子和神经肽可能是应激时神经内分泌免疫系统的信息通道。在本研究中，我们对年轻成年C3H/He小鼠进行了1-5天的食物限制，研究了各种免疫参数的一系列变化，以及一些内源性应激相关体液因子的水平，以阐明它们在急性食物限制中的作用。每天禁食23小时，持续1-5天，小鼠胃溃疡、脾脏和胸腺萎缩，并伴有血浆皮质醇和儿茶酚胺水平升高。它还调节了脾脏的几种免疫细胞功能，包括B细胞数量减少但B细胞有丝分裂原反应没有变化，T细胞数量增加但L3T4/ lyt2比值没有变化，巨噬细胞自然杀伤活性和O_2^-产生的早期增加。这些影响被认为与压力相关的体液因子的增加有关，这可能部分是由于食物限制引起的压力。近年来，自由基介导的胃黏膜损伤被认为与应激有关。我们研究了胃粘膜中抗氧化酶的活性，以阐明自由基在应激反应中的作用。各酶活性中，在急性胃粘膜病变发生前，只有SOD活性下降。这些活动规律可能在AGNL的进展中起一定的作用。

Acute food restriction has been thought to induce psychosomatic stress in man and has been shown to develop stress ulcers in murine stomach. Recently, increasing evidence has been found which suggests that stress hormones, some lymphokines and neuropeptidesmay represent an information channel in the neuro-endocrine-immune system in stress. In the present study we imposed food restriction on young adult, C3H/He mice, for 1-5 days and studied the serial changes of various immune parameters, together with the endogenous level of some stressassociated humoral factors to clarify their implications in acute food restriction. Daily 23-h food deprivation for 1-5 days induced gastric ulcers and atrophic changes of the splean and thymus, accompanied by a rise inplasma cortisol and catecholamine levels in mice. It also modulated several immune cell functions in the splean including a drop in the B cell population but no change in the mitogen response of the B cells, an increase in T cell population but no change in the L3T4/Lyt 2 ratio and an early increase in natural killer activity and O_2^- production by macrophages. These effects are thought to correlate to the increase in stress-associated humoral factors and this may partly result from stress induced by food restriction. Recently, free radical-mediated injury of gastric mucosa has been suggested to be involved in some sort of stress. We studied the activities of antioxidant enzymes in gastric mucosa to clarify the role of free radicals in stress-induced response. Among the enzyme activities, only SOD activity was decreased before the development of acute gastric mucosal lesion. The laws of the activity may play some role in the progress of AGNL.