Studies on proliferation and differentiation of human erythroblasts by phase-contrast cinemicrography

1.脐带血来源的人红细胞的研究(1)压缩效应：对红细胞施加适当的压缩，以增强其精细结构的可视化，不影响其增殖和分化的能力。(2)用Soret条带显微摄影术定性地观察了分化的细胞质血红素化的特征。细胞大小、移动和去核也被证明是红细胞分化的有用指标。先天性红细胞生成障碍性贫血III型红细胞的研究CDA III型红细胞大部分表现出与正常红细胞相似的增殖和分化。发育不良的红细胞的形态发生表现为以下两种进展模式：(1)更常见的是，形态发生始于未成熟的红细胞核内出现一个界限清晰的区域。这种清晰的区域在子代细胞和孙子代细胞…中重新出现。有丝分裂后较多。在后者，清晰区的频率、大小和硬度增加，占据了核的大部分，并将其区域化。在这些细胞中出现了终末分化，如最大的血红素化和剧烈的运动，细胞核变成小叶。这些细胞似乎属于末代细胞，因为它们显示了生长能力，并达到了大多数CDA III红细胞分裂的细胞大小。然而，他们并没有分裂。我们推测这一序列可能最终导致CDA III中最常见的畸形红细胞类型，即大的正染正常母细胞，具有小叶或节段性核，DNA含量从2C到4C。(2)较少见的是，有丝分裂和随后的细胞行为异常，如三极或双极有丝分裂和随后的子细胞融合，或一个子细胞快速生长伴随另一个子细胞的萎缩。这些过程发生在细胞死亡之前。这一过程明显地产生了巨大的红细胞和一些双核红细胞。较少

1. Studies on human erythroblasts derived from cord blood(1) Effect of compression : Proper compression applied to erythroblasts to enhance visualization of their fine structures was proved to affect no influence on their capability to proliferate and differentiate.(2) Characterization of differentiation Cytoplasmic hemoglobinization was qualitatively surveyed by Soret band micrography. Cell size, movement and enucleation were also proved to be useful indices of differntiation of erythroblasts.2. Studies on Congenital dyserythropoietic anemia type III (CDA III) erythroblasts The majority of CDA III erythroblasts showed proliferation and differntiation similar to that of normal erythroblasts. Morphogenesis of dysplastic erythroblasts showed the 2 patterns of progression outlined below.(1) More frequently, morphogenesis began with the appearance of a well demarcated clear area in the nucleus of immature erythroblasts. This clear area reappeared in the daughter cells and granddaughter cel … More ls after mitosis. In the latter, the clear area, which increased in frequency, size and rigidity, occupied most of and compartmentalized the nucleus. Terminal differentiation, such as maximal hemoglobinization and vigorous movement, appeared in these cells and the nucleus became lobular. These cells seemed to belong to the end cell generation, because they showed the capacity to grow and attained the cell size at which most CDA III erythroblasts would divide. However, they did not divide. We speculate that this sequence could culminate in the most common type of dysmorphic erythroblasts in CDA III, the large orthochromatic normoblasts with lobular or segmented nucleus with DNA content ranging from 2C to 4C.(2) Less frequently, abnormalities of mitosis and subsequent cellular behavior, such as tri- or bi-polar mitosis and subsequent fusion of the daughter cells or rapid growth of one daughter cell concomitamt with shrinkage of the other daughter cell were noted. These process preceded cell death. Giant erythroblasts and some binucleate erythroblasts were apparently yielded by this process. Less