Antitumor Substances of the Mucilage-secrete Plants in W.U.S.A.

研究了1991年夏季从俄勒冈州西南部和加利福尼亚州西北部采集的3种地衣和83种高等植物（82种精生植物和1种蕨类植物）（含46种表面有分泌物的植物）的丙酮、乙醇和/或70%水乙醇提取物对HIV-1逆转录酶（RT）的抑制作用、对eb病毒（EBV）激活的抑制作用以及对培养的P-388癌细胞的细胞毒活性。46种植物对HIV-1 RT均有明显的抑制作用，其中大黄蓼、三叉戟、海参、格林利亚等2种植物的乙醇提取物在0.5 mug/ml浓度下的抑制作用大于20%。此外，来自Purshia tridentata的β -谷甾醇葡萄糖苷（IC_<50>=1.8 mug/ml）和来自Amelanchier alnifolia var. pumila的熊果酸和白桦果酸（IC_<50>=1.4和0.8 mug/ml）对HIV-1 RT有明显的抑制作用. ...更多的15种（Eriodictyon californicum等）的丙酮提取物和47种（Sophora leachiana等）的乙醇提取物对EBV的激活有显著的抑制作用。此外,3黄烷酮类(5、7 - (OH) _2黄烷酮等)分离大肠californicum和4黄酮醇(ermarin等)从Cordylanthus viscidus EBV激活了有效的抑制。研究了115种提取物（57种）对P-388细胞的细胞毒活性，但目前还没有得到理想的结果。同样，1992年夏季从俄勒冈州东南部收集的一种地衣和162种高等植物（160种精子植物和2种蕨类植物）的丙酮、乙醇（或甲醇）和/或70%水乙醇（或甲醇）提取物也进行了上述三种生物试验。迄今为止，已发现9种荆刺、金缕梅等3种植物提取物对HIV-1 RT有较强的抑制作用，14种荆刺、樱桃等10种植物提取物对EBV的激活有较强的抑制作用。少

Acetone, ethanol, and/or 70% aqueous ethanol extracts of 3 lichens and 83 species of higher plants (82 Spermatophytes and a Pteridophyte) (containing 46 species of plants with surface exudates), collected from SW Oregon and NW California in Summer of 1991, were tested for their inhibitory effects on HIV-1 reverse transcriptase (RT), their inhibitory effects on the Epstein-Barr virus (EBV) activation, and their cytotoxic activities against cultured P-388 cancer cells, respectively.Significant inhibitory effects on HIV-1 RT were observed in 46 species and especially, ethanol extracts of polygonum majos, Purshia tridentata, Ceanothus integerrimus, Grindelia nana and other 2 species showed more potent inhibition than 20% at 0.5 mug/ml concentration. Furthermore, beta-sitosterol glucoside (IC_<50>=1.8 mug/ml) isolated from Purshia tridentata, and ursolic and betulinic acids (IC_<50>=1.4 and 0.8 mug/ml,respectively) from Amelanchier alnifolia var. pumila showed potent inhibition on HIV-1 RT. … More Acetone extracts of 15 species (Eriodictyon californicum and others) and ethanol extracts of 47 species (Sophora leachiana and others) showed significant inhibition on the EBV activation. Furthermore, 3 flavanones [5,7-(OH)_2 flavanone, etc.] isolated from E. californicum and 4 flavonols (ermarin, etc.) from Cordylanthus viscidus gave potent inhibition on the EBV activation.Cytotoxic activities against P-388 cells were examined in 115 extracts (57 species) which are giving no desirable results at present.Similarly, acetone, ethanol (or methanol), and/or 70% aqueous ethanol (or methanol) extracts of a lichen and 162 species of higher plants (160 Spermatophytes and 2 Pteridophytes), collected from SE Oregon in Summer of 1992 have been subjected to the before-mentioned three types of the biological tests. Up to date, it has been revealed that 9 extracts from Rosa woodsii, Ribes aureum, and other 3 species showed potent inhibitory activities on HIV-1 RT and 14 extracts from Pentagrama triangularis, Prunus emarginata and other 10 species, potent inhibitory effects on the EBV activation. Less