A comprehensive study on plant molecular systematics in the higher plants based on organellar DNA analyzes

教授在本财年内完成了这项工作。在这三年中，与会者进行了卓有成效的合作学习，召开了会议和座谈会。(1)研究方法。为了更方便地获得被子植物的分子信息，我们尝试检测了用聚合酶链式反应方法扩增的叶绿体DNA的几个区域的限制性内切酶位点变异。(2)基于叶绿体DNA的RFLP分析和rbol基因序列分析，对木通科植物的叶绿体DNA进行了分子系统发育研究。两种方法获得的系统发育树的差异归因于检测到的信息性特征的数量。(清水、上田和山口)。经RFLP分析，证实有10个二倍体属起源于多倍体属(n=17，25)。同样，卡里默斯属被证明是多系起源的，卵形紫薇被发现是o…杂交种。Aster和Kalimeris之间更严谨，以Aster为母本植物(Ito等人)。研究了不同栽培植物的变异和起源，特别参考了荞麦(Kishima等人)。Sesamum(Yamada等人)、马铃薯(Hosaka等人)(3)线粒体DNA通过对cox11和atp6基因的RFLP分析，检测到5种线粒体基因组图谱。在北海道南部和东北地区北部，它的变异性比其他地区更大，这表明在那里栽培的大头参的起源。栽培苹果Beta的变异和起源(均为Mikami等人)。Oryza(Kadowaki等人)和小麦属(Mori et al.)1993年9月，Terachi在日本遗传学会第65届会议上举办了题为“高等植物的分子系统学”的研讨会。通过本项目获得的所有结果将发表在一本名为《基于细胞器DNA分析的高等植物分子系统学综合研究》的书中。较少

The profect has finished within this fiscal year. During these three years, the participants have made a fruitful co-operative study, holding assemblies and symposia.(1)Research method. For an casier way to obtain molecular information of the angiosperms, we attempted to detect restriction site variations in several regions of chloroplast DNA amplified by PCR method. The research example was shown in the Rosales(Terachi, Shimizu et al.).(2)Chloroplast DNA The molecular phylogeny in the Lardizabalaceae based on RFLP analysis of chloroplast DNA and also on sequence of rboL gene. The difference in the phylogenetic trees obtained between both methods was attributable to numbers of informative characters detected. (Shimizu, Ueda and Yamaguchi). On the Eupatorieae, the diploid genera (n=10) were proved originated from polyploid genera (n=17,25) based on RFLP analysis (Yahara et al.). Likewise, the genus Kalimeris was proved of polyphyletic origin, and Aster ovatus was found to be of hybrid o … More rigin between Aster and Kalimeris with Aster as maternal plants (Ito et al.). Variation and origin of various cultivated plants were studied with special referensces to Fagopyrum (Kishima et al.). Sesamum (Yamada et al.), potatoes (Hosaka et al.) and Triticum (Terachi et al.).(3)Mitochondrial DNA Five patterns of mitochondrial genome were detected through RFLP analysis of coxll and atp6 genes. In southern Hokkaido and northern Tohoku district, it is more variable than in other areas, suggesting origin of cultivated G.max there 8Mikami et al.). Variation and origin of the cultivated Beta, Malus(both Mikami et al.). Oryza(Kadowaki et al.) and Triticum(Mori et al.) were also discussed.In September 1993, a symposium entitled "Molecular systematics on the higher plants" organized by Terachi was held at the 65th Meeting of The Genetics Society of Japan. All the results obtained through the present project will be published in a book entitled "A comprehensive study on plant molecular systematics in the higher plants based on organellar DNA analyzes". Less