Studies on immunostimulating ability and active components in lactic acid bacteria used to fermented milk

德尔布鲁氏乳杆菌亚种细胞壁成分的研究。用溶菌酶和n -乙酰神经氨酸酶对保加利亚乳杆菌和嗜酸乳杆菌进行水解鉴定。阐明了两种之间的差异。为了评价嗜酸乳杆菌群乳酸菌对肠道内生长的生物活性，在小鼠腹腔巨噬细胞培养中研究了嗜酸乳杆菌和加色乳杆菌对干扰素的诱导作用。9株嗜酸乳杆菌中的4株在巨噬细胞培养中诱导干扰素。在13株菌株中，有5株菌株也产生了这种激活作用。用抗干扰素血清处理诱导的干扰素表现为α / β。采用小鼠脾细胞有丝分裂活性法对9株嗜酸乳杆菌的全细胞和水解细胞组分进行了生物活性评价。6株菌株的细胞壁成分均表现出较高的活性。用离子交换色谱法对其中一个菌株的细胞壁组分进行分离，然后进行活性测定和化学分析。活性主要存在于结合部位，并证实对小鼠脾细胞b细胞有特异性。活性组分的碳水化合物部分由葡萄糖、n -乙酰氨基葡萄糖、n -乙酰氨基乙酸和甘油组成。较高的活性组分往往含有较高的n -乙酰氨基葡萄糖与葡萄糖的比例。结果表明，某些嗜酸乳杆菌群菌株在食用含有该乳酸菌的乳制品时，可在肠道内发挥免疫刺激作用。

The cell wall components of L. delbrueckii subsp. bulgaricus and L.acidophilus were characterized after hydrolysis with lysozyme and N-acetylneuraminidase. The difference between two species were elucidated.In order to evaluate the biological activity induced by L. acidophilus group lactic acid bacteria which can grow in intestinal tract, induction of interferon by L. acidophilus and L. gasseri was investigated in murine peritoneal macrophage cultures. Four strains in 9 L. acidophilus strains induced interferon in macrophage cultures. Such activation effect by L. gasseri was also formed on 5 strains in 13 strains. Induced interferons were characterized as alpha/beta by treatment with anti-interferon serum.The biological activities of whole cell and hydrolyzed cell component of 9 L. acidophilus strains were evaluated by mitogenic activities using murine spleen cells. Higher activity was observed in only one whole cell, however, the cell wall components from 6 strains showed significantly higher activity. The cell wall component from one strain which showed the highest value was fractionated by ion-exchange chromatography, followed by measurement of the activity and chemical analysis. The activity was mainly found in the bound fractions and it was confirmed to be specific on B-cell of murine spleen cells. Carbohydrate moiety of the active fractions was composed of glucose, N-acetylglucosamine, N-acetylmuramic acid and glycerol. Higher active fraction tends to contain higher N-acetylglucosamine in the ratio against glucose.The results indicate that some strains of L. acidophilus group can act as an immunostimulators in the intestinal tract when dairy products containing such lactic acid bacteria was ingested.