Diagnosis of Rejection in Renal Transplantation Recipient by Reactivity of Peripheral Mononuclear Cells to IL-2

白细胞介素-2 （IL-2）被认为能引起引起同种异体移植排斥反应的细胞的增殖。通过检测外周血单个核细胞（PBMN）对重组白细胞介素-2 （IL-2）的反应，监测同种异体肾移植受者的宿主免疫状态。24例患者处于稳定状态。11名患者在发生排斥反应时接受了研究。8例患者分别在稳定状态和排斥状态下进行了研究。同时选取9例健康对照和7例慢性肾功能衰竭血液透析患者进行比较。以2.5 U/ml （IL-2-LR）浓度的il -2孵育90小时后，通过摄取^3H来评价PBMN的反应。此外，以K562、Daudi和供体PBL为靶细胞，用Cr^<51 bb0 Cr释放法测定扩增细胞的细胞毒性。为了研究参与该反应的细胞的特征，采用双色流式细胞术对细胞表面标记物进行了多种组合分析。

Interleukin-2 (IL-2) is thought to evoke potent expansion of cells which are responsible for allograft rejection. The response of peripheral blood mononuclear cells(PBMN) to recombinant IL-2 (rIL-2) was examined in order to monitor the host immunological state in renal allograft recipients. Twenty-four patients were studied at the stable state. Eleven patients were studied at the time of rejection. Eight patients were studied both at the stable state and at the rejection. In addition, 9 healthy controls and 7 patients under hemodialysis for chronic renal failure were also studied for comparison. The response of PBMN were evaluated by ^3H uptake after a 90-hour-incubation with rIl-2 at the concentration of 2.5 U/ml (IL-2-LR). In addition, Cytotoxicity of the expanded cells were measured by Cr^<51>Cr release using K562, Daudi and donor PBL as target cells. In order to investigate the characteristics of cells participating in this reaction, the surface markers of cells were analyzed by two-color flow cytometric evaluation using several combinations.