Cellular Responses of Human Periodontal Ligament Fibroblasts by the Mechanical Stress

牙周组织总是受到咀嚼或咬合的压力，这可能表现为几种类型的机械应力。在这项研究中，我们主要研究了Kawase，T等人(1988)所描述的人第一前磨牙牙周韧带成纤维细胞的细胞生长和分化以及细胞附着和扩散因子(CASFS)。通过机械拉伸显着合成了可能促进牙周组织再生的CASFS。通过以下实验对CASFS的特性进行了研究。结论：1.含RGD细胞附着信号域的Gly-Arg-Asp-Ser多肽不能抑制HPLF(HPLF-CM)条件培养液中的CASFs。HPLF-CM的CASFs不影响抗纤维连接蛋白(FN)、玻璃体连接蛋白(VN)及其受体的多克隆抗体，并能与羟基磷脂结合。HPLF-CM不仅含有CASFS，还含有受生长因子调控的CASF活性抑制物，说明机械应力可能在细胞的增殖和分化中起重要作用，也可能调节细胞外基质的产生。

Periodontium is always stressed by the mastication or oclussion which might be expressed with the several categories of mechanical stresses. In this research project, we have focuesed on the cell growth and differentiation, and cell attachment and spreading factors (CASFs) of the fibroblasts derived from human periodontal ligaments of first premolars as descrived by Kawase, T et al (1988). The CASFs which may promote the regeneration of periodontium were dramatically synthesized by the mechanical stretching. The characterization of CASFs were investgated by the following experiments.1. The CASFs in the conditioned medium of HPLF(HPLF-CM) were not inhibited by Gly-Arg-Asp-Ser peptide containing RGD cell attachment signal domain.2. The CASFs of HPLF-CM did not affect polyclonal antibodies against fibronectin(FN), vitronectin(VN) and their receptors and could bind with hydroxyapatite.3. The HPLF-CM did not contain only CASFs, but also inhibitors to CASF activity which were regulated by growth factors.We conclude that mechanical stress may play an important role in the cell proliferation and differentiation, and also could regulate the production of extracellular matrix such as CASFs.