Studies of the proton motif force (PMF) in the cyanobacterium Synechocystis with the the aim to maximize photosynthetic hydrogen production

在光合作用中，光被捕获并用于水分解。这导致释放的电子通过电子转移链转移，用于产生NADPH和ATP。NADPH和ATP随后被Calvin-Benson-Bassham（CBB）循环用于CO2固定和碳水化合物的合成。在这个项目中，我们的目标是量化的速率，电子流通过光系统II（PSII）和光系统I（PSI）在并行O2和CO2营业额的蓝藻集胞藻属PCC 6803。收集的数据将提供所涉及的光合电子传递途径的图片，产生的ATP和NADPH的量，因此也是质子基序力（PMF）的措施。关于PMF形成及其在细胞中可能的耗散的知识对于将来在生物制氢方面使用光合电子传递是必不可少的。如果来自水氧化的大部分电子在PSI转移到H2生产，则与NADPH相比PMF形成的不平衡是预定的。为了最大限度地提高光合氢的生产和发展一个持久的过程，必须耗散部分PMF。

In photosynthesis light is captured and utilized for water splitting. This results in the transfer of liberated electrons through an electron transfer chain for the production of NADPH and ATP. NADPH and ATP are subsequently utilized by the Calvin-Benson-Bassham (CBB) cycle for CO2 fixation and the synthesis of carbohydrates. In this project we aim to quantify the rates of electron flow through photosystem II (PSII) and photosystem I (PSI) in parallel to O2 and CO2 turnover in the cyanobacterium Synechocystis sp. PCC 6803. The collected data will deliver a picture of the involved photosynthetic electron transport routes, the amount of ATP and NADPH produced and consequently also a measure of the proton motif force (PMF) in play. Knowledge on PMF formation and its possible dissipation in the cells is essential in respect to any future use of the photosynthetic electron transport in terms of biological hydrogen production. If a large proportion of the electrons derived from water oxidation is diverted at PSI to H2 production an imbalance of PMF formation compared to NADPH is predetermined. To maximize photosynthetic hydrogen production and to develop a persisting process, it is imperative to dissipate part of the PMF.