Genetic flux between chloroplast and mitochondria genomes in Triticum and Aegilops.

在许多植物中已经报道了植物细胞器之间的遗传通量。为了研究小麦群体叶绿体基因组和线粒体基因组间遗传物质易位的相关问题，我们在普通小麦的线粒体DNA主拷贝上对易位的叶绿体基因进行了物理定位，并通过探测易位的叶绿体基因对小麦和藜麦的线粒体DNA进行了RFLP分析。利用PCR技术构建了20个叶绿体基因的特异性探针。定位数据显示，在线粒体基因组中检测到与9个叶绿体基因同源的序列。9个叶绿体基因中，有3个在线粒体中具有转录活性。用叶绿体atpA和psaA基因对14个小麦异质系的线粒体dna进行杂交分析，发现这两个基因的RFLP具有较高的变异性。基于杂交片段相似性，估计了系统发育关系。14种血浆类型间的亲缘关系因个体基因而异。

Genetic fluxes among plant organella have been reported in many plants. To investigate aspects of the translocation of genetic material between chloroplast and mitochondrial genomes in the wheat group, we carried out physical mapping of the translocated chloroplast genes on the master copy of mitochondrial DNA of common wheat, and RFLP analysis of mitochondrial DNAs in Triticum and Aegilops by probing translocated chloroplast genes. Specific probes for 20 chloroplast genes were constructed by PCR. Mapping data show that homologous sequences with the nine chloroplast genes, were detected in the mitochondria genome. of nine chloroplast genes, three were transcription activity in the mitochondria as detected by Northern hybridizaition. Hybridization profiles of mitochondrial DNAs from 14 alloplasmic lines of wheat probed with chloroplast atpA and psaA genes revealed high variabilities of RFLP for both genes. Based on the hybridized fragment similarity, phylogenetic relationships were estimated. Different relatedness among 14 plasma types were obtained depending on individual genes.