Interaction between the substratum and mesenchyme and regulation by growth factor in mammary glands and reproductive organs.

1) MAP激酶参与信号转导：胰岛素、IGF-I或EGF中的一种对乳腺生长至关重要。MAP激酶存在于乳腺上皮细胞中。2)泌乳素受体表达的调控：乳腺中泌乳素受体受糖皮质激素诱导。竞争性PCR方法在转录水平上证实了这种诱导作用。3) HC11对催乳素的反应性：HC11是一种来源于正常乳腺上皮细胞的细胞系，对催乳素的反应非常差。为了提高反应性，将长型催乳素受体cDNA导入HC11细胞。目前还没有获得高响应克隆。4)肝细胞生长因子对乳腺肿瘤生长的影响：Baculo病毒载体系统调控成纤维细胞所含的一种生长因子。针对该蛋白的抗体抑制肿瘤细胞生长。5)从p53缺陷小鼠组织中建立细胞系：从神经、精囊和乳腺中建立了许多细胞系。6) EGF对早期胚胎葡萄糖掺入的影响：早期胚胎不能利用葡萄糖，因为己糖激酶直到囊胚阶段才表达。GLUT 1是一种促进葡萄糖结合的蛋白，首次在囊胚阶段表达。EGF刺激这种表达。7) IGF-I及其结合蛋白对黄体功能的调控：采用点印迹法和northern印迹法研究黄体中IGF-I和IGFBP3的表达。igf - 1升高直至分娩。

1) Involvement of MAP kinase in signal transduction : One of insulin, IGF-I or EGF is essential for mammary growth. MAP kinase exists in mammary epithelial cells. 2) Regulation of the expression of prolactin receptor : Prolactin receptor is induced by glucocorticoids in the mammary gland. This induction was demonstrated by the competitive PCR method at the level of transcription. 3) Responsiveness of HC11 to prolactin : HC11, a cell line derived from normal mammary epithelial cells, responses very poorly to prolactin. To improve the responsiveness, cDNA for long-form prolactin receptor was introduced to HC11 cells. No highly responsive clone was obtained yet. 4) Effect of hepatocyte growth factor on mammary tumour growth : A growth factor contained by fibroblast conditioned by the Baculo virus vector system. Antibody against the protein inhibits tumour cell growth. 5) Estasblishment of cell lines from tissues of p53-deficient mice : Many cell lines were established from nerves, seminal vesicles and mammary glands. 6) Effect of EGF on glucose incorporation in early embryos : Early embryos cannnot utilize glucose becouse hexokinase is not expressed until the blastocyst stage. GLUT 1, a protein which promotes glucose incorporation, expresses at the blastocyst stage for the first time. EGF stimulates this expression. 7) Regulation of luteal function by IGF-I and its binding proteins : To investigate the regulation, expressions of IGF-I and IGFBP3 in corpora lutea were investigated by dot blotting and northern blotting analysis. IGF-I increased until parturition.