Studies on the vegetative propagation and the artificial seed production in the mulberry by tissue culture methods

为适应桑树生物技术的发展，进行了桑树生物技术引种栽培和人工种子生产试验。随后，对主要课题进行了有意义的研究，大西等报道了从愈伤组织中诱导出不定胚和不定芽的成功结果。在这些研究之后，他研究了在种子胶囊中含有营养物质的培养基中对种子萌发和生长的各种影响以及播种种子的培养基的变化。在此基础上，研究了桑树人工种子的生产和贮藏方法，并研究了Cd、Cu、Zn、Ni对桑树愈伤组织生长的耐性和Mn与Cd共存对桑树愈伤组织生长的抑制作用。在继代培养中获得了具有重金属耐性的愈伤组织。的rela ...更多信息 结果表明，重金属的吸收浓度与处理浓度之间存在一定的关系，Mn对愈伤组织的生长抑制作用大于Cd对愈伤组织的生长抑制作用，Cd和Mn的积累量也大于Cd。Kuno等报道了雌性品种在生育后期皮层中有大量铁积累的趋势，此外，Fe和Co的添加还促进了雌性器官之一的核的生长。Oshigane对不同品种愈伤组织的增殖和再分化以及氮源对不定芽形成的影响进行了比较观察。用Yanagisawa法测定了桑树种子萌发过程中抑制和促进侧芽萌发的物质及细胞分裂素含量的变化。基于这些结果，可以在一定程度上推测桑树提取物的添加量促进了人工种子愈伤组织的生长。目前，添加量与发芽速度之间的关系正在研究中。在含4PU的液体培养基中进行垂直旋转培养，获得了多芽体的诱导，并阐明了其组织结构。可以通过使用与用于诱导的培养基相同的培养基进行多个芽体的继代培养，并且当多个芽体移植到固体培养基时再生芽。少

By an attempt to the development in the biotechnology of the mulberry, its introduction to the cultivation of the mulberry and examinations on the artificial seed production were carried out. And then, following significant studies to the main subjects writen on a title were mad.Sucessful results on introductions of advetitious embryoes and shoots from the callus were reported by Ohnishi. Following these studies, he examined various influences on germination and growth of seeds in culture media containing nutrients in the capsules of seeds and variations of culture media for sown seeds. An then, the production of artificial seeds was examined and storage methods were developed, based upon previous examinations.Examinations on influences on the tolenrance to Cd, Cu, Zn, Ni to the growth of the callus of the mulberry and the coexistence of Mn to the growth inhibition with Cd were made by Homma. Results on the callus having the heavy metal tolerance in the subculture were obtained. A rela … More tionship between absorbed heavy metal densities and treated densities were recognized.The increased growth inhibition of the callus with the coexistence of Mn was found compared with the growth inhibition of the callus by Cd and amounts of accumulation of the both, Cd and Mn, was found. A tendency of the accumulation of much Fe in the cortex of the latter half of its growth period in the female variety was reported by Kuno and furthermore, promotion of the growth in a nucleous, one of female organs, was recognized with adding of Fe and Co. Comparative observations in varieties on propagation and redifferentiation of the callus and influences of nitrogen sources to the formation of adventitious buds were examined by Oshigane. Changes in contents inhibiting and promoting on germination of lateal bads and in contents of cytokinin in germinating seeds of the mulberry were examined by Yanagisawa. Based upon those results, adding amounts of the extract of the mulberry that promoted the growth of the callus for the artificial seeds were able to be presumed in some extents. At present, a relationship between the adding amount and the germinating velocity is examining. By the vertical rotary culture of seedlings in the liquid culture media containing 4PU,Oka obtained the induction of multiple bud bodies and he also clarified their structures of the tissue. It is possible to make subcultures of the multiple bud bodies by using the same culture medium with the medium for the induction, and shoots were reproduced when the multiple bud bodies were transplanted to solid culture media. Less