c-kit Mutant Rats as a Tool for Mast Cell Research-Development and Function

肥大细胞作为一种效应细胞，在直接超敏反应中起着重要的作用。在工业化国家，由于立即过敏反应而引起的疾病，如过敏性鼻炎、婴儿哮喘和特应性皮炎正在增加。为了分析肥大细胞在即时超敏反应中的作用，肥大细胞缺陷动物是有用的。由于我们最近发现了肥大细胞缺陷的Ws/Ws大鼠，我们试图通过使用Ws/Ws大鼠与各国的肥大细胞研究人员合作。因为最初的Ws/Ws大鼠不是近亲繁殖的。我们试图将Ws突变基因引入某些近交系。然而，我们没有找到合适的近交系，在这种遗传背景下可以饲养相当数量的Ws/Ws大鼠。因此，我们将封闭菌落的Ws/Ws大鼠清洗到特定的无病原体（SPF）状态。将实验动物送往国外需要SPF条件。由于在SPF条件下生产这种w / w大鼠是令人满意的，我们现在计划将这些w / w和对照+/+大鼠送到哈佛大学、马斯特大学和爱丁堡大学。我们认识到，获得可以运往国外的有用的实验动物花了相当长的时间。辻村参观了爱丁堡大学，了解了一项关于曼氏血吸虫感染的实验。Kitamura拜访了哈佛大学，解释了SPF条件下Ws/Ws大鼠生产延迟的原因。加利、珀杜和威斯特林（代表米勒）参观了大阪大学，了解了w / w鼠的性状和育种方法。我们一起讨论了未来使用Ws/Ws大鼠的合作。

Mast cells play an important role for immediate hypersensitivity reaction as an effector cell. Deseases that are induced as a result of immediate hypersensitivity reaction such as allergic rhinitis, infantile asthma and atopic dermatitis are increasing in industrialized countries. To analize the role of mast cells in immediate hyper-sensitivity reaction, mast cell-deficient animals are useful. Since we recently found mast cell-deficient Ws/Ws rats, we attempted to colaborate with mast cell researchers of various counties by using Ws/Ws rats. Since the original Ws/Ws rats are not inbred. we attempted to introduce the Ws mutant gene into certain inbred strains. However, we cound not find any suitable inbred strains, under which genetic background appreciable number of Ws/Ws rats could be raised. Therefore, we cleaned Ws/Ws rats of closed colony into specific pathogen-free (SPF) condition. SPF condition is required to send experimental animals to foreign countires. Since production of such Ws/Ws rats in the SPF condition appeared to be satisfactory, we are now planning to send these Ws/Ws andcontrol +/+ rats to Harvard, MaMaster and Edingurgh Universities. We recognized that it took rather long period to obtain useful experimental animals that can be transported to the foreign countires. Tsujimura visited Edinburgh Univeirsty to learn about an infection experiment with Shistomsoma mansoni. Kitamura visited Harvard University to explain the delay of the production of Ws/Ws rats in SPF condition. Galli, Perdue and Westling (on behalf of Miller) visited Osaka University to learn the characters and the breeding method of Ws/Wsrats. We all to gether discussed future colaboration by using Ws/Ws rats.