The absorption study with BMIPP in a swine model of partial small intestinal transplantation

本研究旨在建立一种新的猪小肠移植模型，并应用BMIPP**&gt；**&gt；评估移植物的功能。第一阶段：对体重10-15 kg的小型猪进行自体部分小肠移植，以探讨该手术技术的可行性，并确定最合适的肠段和长度。原位部分回肠移植是可行的。在这个模型中，超过30%的小肠全长是实现长期存活所必需的。第二阶段同种异体移植研究同种异体移植采用与第一阶段研究相同的技术。免疫抑制方案包括FK506(0.3 mg/kg/d)和甲基强的松龙。BMIPP的时相吸收研究BMIPP在自体或同种异体部分回肠移植中的移植物吸收研究。结果与对照组动物的结果进行了比较。观察长链脂肪酸吸收功能的可逆性。然而，不同动物的吸收功能恢复情况差异很大，需要进一步研究以确定BMIPP在该模型中的临床意义。

This study was designed to establish a new swine model of small intestinal transplantation and to evaluate the graft function using BMIPP^<**>.Phase 1 Autotransplantation studyAutotransplantation of partial intestine was performed in small swine (body weight, 10-15kg) to elucidate the feasibility of the operative technique and to decide the most appropriate part and length of the intestine. Orthotopic partial ileal transplanation was proved to be feasible. More than 30% of the total length of the small intestine was necessary to achieve long-term survival in this model.Phase 2 Allotransplantation studyAllotransplantation was performed in the same technique as in phase 1 study. A immunosuppression protocol consisting of FK506 (0.3mg/kg/day) and methyl prednisolone was employed. One-month survival of the recipients was achieved and the graft function test was possible in this model.Phase Absorption study with BMIPPThe graft absorption of BMIPP was studied in recipients which underwent auto-or allotransplantation of partial ileum. The results were compared with those in control animals. The reversibility of absorptive function of long-chain fatty acids was observed. However, the recovery of absorptive function was so different among animals that further study is needed to decide the clinical significance of BMIPP in this model.^<**> : BMIPP,^<123>I-Iodophenylmethylpentadecanate