Development of highly sensitive analytical system for biologically active hydroxylated fatty acids with a new fluorescent derivatization reagent, AE-OTf

我们开发了一种高灵敏度的荧光衍生试剂AE-OTf [2-（2，3-蒽二甲酰亚胺基）乙基三氟甲磺酸酯]，它将是迄今为止制备的羧酸衍生试剂中最灵敏的试剂。利用AE-OTf，我们研究了脂肪酸和羟基化脂肪酸（如藜芦碱）分析体系的发展。首先，我们成功地开发了亚毫微微摩尔等度洗脱模式的脂肪酸高效液相色谱分析系统。该系统已由JASCO集团改进为自动进样系统。接下来，开发了一种新的LC-LC柱切换系统，用于分析具有羟基的复杂羧酸，如甲壳类贝类毒素;冈田酸（OA），甲藻毒素-1（DTX-I）和甲藻毒素-3（DTX-III）。通过该系统，每种毒素可以被确定为皮克水平。该分析符合 ...更多信息 该法被许多分析化学家推荐为WHO的官方分析方法。成功的结果与贝类毒素，复杂的羧酸与羟基基团，促使我们开发一个分析系统的香茶菜。我们已成功地测定了皮克级的标准香豆素类化合物，并正在研究建立血栓烷B_2的自动分析系统，这对临床有重要意义。由于存在许多生物学上重要的手性羧酸，我们的注意力接下来被导向通过修饰AE-OTf来开发用于手性羧酸的高灵敏度手性荧光标记试剂。我们开发了一种新的手性荧光标记试剂（S）-（+）-2-（2，3-蒽二羧基）-1-丙基三氟甲磺酸酯（AP-OTf），并建立了等离子体中贝前列素钠的HPLC分析体系。所有四种异构体都可以通过该系统在皮克水平上进行测定。综上所述，我们开发了用于羧酸的高灵敏度荧光标记试剂和使用这些试剂的皮克级HPLC分析系统Less

We have developed a highly sensitive fluorescent derivatization reagent, AE-OTf [2-(2,3-anthracenedicarboximido)ethyl trifluoromethanesulfonate] which will be the most sensitive reagent in the world among the reagents so far prepared, for carboxylic acids. By using AE-OTf, we have studied the development of analytical system for both fatty acids and hydroxylated fatty acids (such as prostagrandines). At first, we have succeeded in development of the subfemto molar HPLC-analydicalsystem for fatty acids which are usually encountered in foods and living bodies by isocratic elution mode. This system has been improved by JASCO group to the system with automatic sampler. Next, a new LC-LC column switching system for the analysis of complex carboxylic acids with hydroxyl groups such as diarrhetic shellfish toxins ; okadaic acid (OA), dinophysistoxin-1 (DTX-I), and dinophysistoxin-3 (DTX-III) has been developed. By the system each toxin can be determined an pico-gram level. This analytical met … More hod is recommended for the official analytical method of WHO by manyl analytical chemists. The successful results with diarrhetic shellfish toxins, complex carboxylic acids with hydroxyl groups, prompted us to develop an analytical system for prostagrandins. We have succeeded in the determination of standard prostagrandins at pico-gram level and are now studying to develop an automatic analytical system of thromboxane B_2 which will be very importand for crinical use. Since there are many biologically important chiral carboxylic acids, our attention was next directed to the development of a highly sensitive chiral fluorescent labeling reagents for chiral carboxylic acids by modifying AE-OTf. We have developed a new chiral fluorescent labeling reagent, (S)-(+)-2-(2,3-anthracenedicarboxido)-1-propyl trifluoromethanesulfonate (AP-OTf) and an HPLC-analytical system for Beraprost sodium in Plasama. All four isomers can be determined at pico-gram level by the system. As summary, we have developed highly sensitive fluorescent labeling reagents for carboxylic acids and pico-gram level determination HPLC-analytical systems using these reagents Less