Laparoscopic surgery and adjuvant chemotherapy based on DNA analysis using fluorescence in situ hybridization in fresh human gastrointestinal tumors

应用荧光原位杂交技术（FISH）检测59例胃癌标本中染色体和p53基因的改变，探讨其作为胃癌潜在恶性标志物的价值及其临床应用。结果表明：1.胃活检组织中存在染色体数目畸变，胃癌组织与癌旁正常组织中的单体和多体性比例无显著性差异; 2.胃癌组织与癌旁正常组织中的1、7、11号染色体单体比例和1、7、11、17号染色体3个拷贝以上的多体性比例有显著性差异。结果提示染色体数目畸变可能参与了肿瘤的发生和发展。3.在31例手术标本中，7、11和17号染色体上的多倍体肿瘤淋巴结转移和淋巴管浸润的发生率明显增高。显著的高发病率 ...更多信息 在17号染色体的多倍体肿瘤中观察到静脉浸润。在1号和11号染色体的非整倍体肿瘤中观察到腹膜播散的显著高发生率。4.应用双色荧光原位杂交技术对20例早期胃癌进行检测，发现胃癌细胞中p53缺失的细胞比例明显高于正常组织。更重要的是，在淋巴结阳性的胃癌中，p53缺失的比例显著增加。5.根据上述结果，胃活检标本双色FISH分析是预测胃癌前病变、胃癌浸润深度、淋巴结转移的有效方法，尤其是对淋巴结转移的预测。少

The efficacy of fluorescence in situ hybridization (FISH) in alteration of chromosome and p53 gene was evaluated as an potential malignant marker and its clinical application in 59 gastric cancer specimens. The results are the followings :1.Numerical chromosome aberrations in gastric biopsy specimens could be detected with FISH.There was no significant difference concerning the fraction of monosomy and polysomy between normal and adjacent normal tissues in gastric tumor specimens.2.There was a significant difference concerning the fraction of monosomy in chromosome 1,7,11 and 3 polysomy (more than 3 copies per cell) in chromosome 1,7,11,17 between normal and tumor tissues. These results indicate that chromosome numerical aberrations may involve in tumorgenesis and its development.3.Regarding 31 surgical specimens, the significant high incidences of lymphnode metastasis and lymphoductal invasion were observed in polyploid tumors in chromosome 7,11 and 17. The significant high incidence … More of venous invasion was observed in polyploid tumors in chromosome 17. The significant high incidence of peritoneal dissemination was observed in aneuploid tumors in chromosome 1 and 11. These results suggest that the specific numerical aberrations of chromosomes may involve in invation and development of gastric tumors.4.Using dual-color fluorescence in situ hybridization 20 early gastric cancers, a significant increase of the fraction of p53 decreased cells was observed in gastric cencer with sm invasion than that of normal tissues. More importantly, a signofocant increase of the fraction pf p53 deletion was observed in lymphnode positive gastric cancers with sm invasion. This finding suggests that lymphnode metastasis may be associated with the deletion of p53 gene in early gastric cancer.5.Based on these results, dual-color FISH analysis with gastric biopsy specimens seems effective method which could predict malignent potential of premalignant lesion, and that of gastric cancers with m and sm invasion before operation, especially concerning lymphnode metastasis. Less