About influence of radiation for cultivation human skin fibroblasts and hormone

检测辐射、类固醇激素、甲状腺激素和辐射对人皮肤培养成纤维细胞的影响。方法：对人皮肤培养成纤维细胞进行传代培养，用传代早期的α细胞进行模拟。辐射照射可精确控制剂量，线形选择~(60)Co伽玛射线，使之恒定，用遥钴仪进行0.3Gymin、5Gy10Gy20Gy线的重复性试验。因为剂量的重复性不是固定在其他导线形式上的，所以我没有用它来模拟。照射后72小时测定α细胞数，照射后24小时评价细胞增殖能力，观察照射后24小时α细胞形态变化，光镜和扫描型/透射电子显微镜观察α细胞形态。其次，0.1、1.0、10.0 mU g/ml分别加入培养液中的甲状腺素(T4)和氢化可的松(HC)，0.7Gymin，…用遥控钴仪进行10Gy射线照射，照射后即刻测量α细胞数，24.48倍，评价细胞增殖能力，24小时后用透射电子显微镜观察α细胞形态。在α细胞形态方面，光镜下未见差异，但扫描电子显微镜下可见微绒毛细胞表面随剂量增加而减少的平坦现象。在透射电子显微镜下，观察残留体形态和线粒体的变化，尤其是低剂量照射24小时后，残留体仍保持在高剂量下。在培养液中加入T4、HC能抑制人皮肤成纤维细胞的生长，而在HC中加入单纯的伽马射线也能抑制成纤维细胞生长。问题：一些差异是通过实验发现更多的认识来煮它，但对应的是单个培养细胞的辐射敏感性的差异，使用相同的同年电荷，原代培养相同的染色体区域。细胞的增殖能力受到辐射的抑制，但由于在短期的观察中没有意识到的变化，所以当想要长期观察而不是未来时，我会考虑阿尔法细胞的形态，当想要做基因水平的分析时也会更多地考虑。较少

I examined influence of radiation, coaction of steroid hormone, thyroid hormone and radiation for human skin cultivation fibroblasts.Method : I did culture passage of human skin cultivation fibroblast, and used alpha cell of passage early stage for simulation. The radiation exposure could control dose precisely, and, in wire form, I selected^<60> Co gamma-ray by it ising possible for constant, and did reproducibllity with 0.3Gy/min, 5Gy.10Gy.20Gy using telecobalt apparatus. Because reproducibility of dose wasn't fixed in other wire form, I didn't use it for simulation. I measured alpha cell number after 72 hours and, after exposure right after, 24 hours, evaluated proliferation of cells ability to examine gamma-ray exposure influence, and observed alpha cell morphology by light microscope and scanning type/transmission electron microscope in exposure right after, after 24 hours.Next, 0.1,1.0,10.0 mu g/ml each added Thyroxine (T4) and Hydrocortison (HC) in culture medium, and 0.7Gy/min, … More 10Gy irradiated gamma-ray using telecobalt apparatus, and I measured alpha cell number after exposure right after, 24.48 time and evaluated proliferation of cells ability, and observed alpha cell morphology by transmission electron microscope in after 24 hours.Consequence : It was accepted an increase of human being cutis cultivation fibroblast to be restrained by gamma-ray exposure. About alpha cell morhology, the differential wasn't found with light microscope, but smoothing of decrement and cell surface of microvilli was looked at with gain of dose with scanning electron microscope. And, in transmission electron microscope, alteration of morphology of gain and mitochondria of residual body was looked at, and, in particular, was looked at with low dose by after 24 hours, and residual body remained with high dose even after 24 hours.That growth of human being cutis cultivation fibroblast was restrained was recognized by adding T4, HC in culture medium than I irradiated a merely gamma-ray in HC too. And, there was the determination which promoted proliferation of cells in T4, and it was thought whether you didn't reduce barrier by radiation exposure.Problems : Some differences are recognized more by experimental findings to boil it, but correspond to a difference of radiation sensitivity of individual cultured cell by using the same year charges, the same chromosome region for primary culture. Proliferation of cells ability is restrained by radiation exposure, but because the alteration which was wasn't recognized in observation between short terms, I think about alpha cell morphology when want to observe a long term than the future, and think when want to do analysis by gene level more too. Less