ENHANCING EFFECTS OF EPIDERMAL GROWTH FACTOR ON HUMAN ORAL SQUAMOUS CELL CARCINOMA INVASION AND METASTASIS.

1)表皮生长因子（EGF）的旁分泌作用为明确表皮生长因子（EGF）对人口腔鳞状细胞癌侵袭能力的影响，对5株细胞系进行了研究。EGF以剂量依赖性的方式增强了所有细胞系的随机运动性，暴露于EGF会增加相同细胞的尿激酶型纤溶酶原激活剂和基质金属蛋白酶-9的产生。这些结果强烈提示EGF可能促进人口腔鳞状细胞癌的侵袭和转移。（中国生物医学工程杂志，17:1668 -175,1996）2) EGF在自分泌方式中的作用在这些细胞系中发现了产生EGF的细胞系。在这些细胞中，肿瘤细胞产生的EGF可以通过EGF受体刺激自身的随机运动。（Oncologia 27: 455-457,1994）3) EGF敏感克隆的建立为了揭示信号通路，我们建立了对EGF刺激最敏感的s-1克隆细胞和最不敏感的i-1克隆细胞。这些克隆细胞的比较可能在不久的将来导致egf诱导运动的信号转导机制。4)卵细胞诱导运动的信号转导分析。当EGF与EGF受体结合时，首先发生酪氨酸磷酸化关系，随后通过erbB同型二聚体引起PLCr的激活和/或erbB3异源二聚体引起pi3激酶的激活，PKC被激活。（JJCR第54届年会论文集，P458）

1) Effect of EGF in paracrine mannerIn order to clear the effects of Epidermal Growth Factor (EGF) on the invasion abilities of human oral squamous cell carcinoma, five cell lines were studied. EGF enhanced the random motility of all cell lines in a dose-dependent fashion and exposure to EGF let to an increased production of urokinase type plasminogen activator and matrix metalloproteniase-9 by the same cells. These results strongly suggest the EGF may promote the invasion and metastasis of human oral squamous cell carcinomas. (Tumor Biology, 17 : 168-175,1996.)2) EFFECT OF EGF IN AUTOCRINE MANNERCell lines, which produced EGF,was found out in these cell lines. In these cells, EGF produced by tumor cells can stimulate the random motility itself through the EGF receptor. (Oncologia 27 : 455-457,1994)3) ESTABLISHMENT OF EGF SENSITIVE CLONETo reveal the signal pathway, we established s-1 clone cells, which was most sensitive clone against the EGF stimulation and also we established i-1 clone cells, which was most insensitive clone. Comparison of these clone cells may lead the mechanisms of signal transduction of EGF-induced motility in near future.4) ANALYSIS OF SIGNAL TRANSDUCTION OF EGF-INDUCED MOTILITY.When EGF bind to the EGF receptor, tyrosine phosphorelation is firstly occurred and subsequently PKC is activated through the activation of PLCr arising from erbB homodimer and/or activation of PI3-kinase arising from erbB3 heterodimer. (Proceeding of JJCR,54th Annual Meeting, P458)