Imaging of dynamic structure of molecular and cellura organization by ultramicroscopic tequniques.

本项目旨在揭示与信号转导相关的细胞骨架或膜骨架的三维动态结构。为此，我们正在开发新的成像技术，包括原子力显微镜、能量过滤电子显微镜和其他改进的视频显微镜。此外，这个项目也致力于揭示蛋白质的分子结构，因为信号转导是基于对每个相关蛋白质的分子识别。也就是说，一系列的分子识别是一个级联的信号转导。显然，分子-分子识别涉及到分子的形状或表面结构。团队中个人的活跃度。项目负责人Usukura先生对这个项目做了报告，同时，他在这一年里尝试对DNA的转录进行成像。sokabe博士通过斑块团法结合视频增强显微镜揭示了机械感觉通道开放与细胞骨架之间的关系。kusumi博士报道了与膜下细胞骨架结构域结构相关的膜蛋白运动调控系统。石川博士用共聚焦显微镜观察了质膜的底涂层结构。木下博士利用新型纳秒多成像显微镜成功地观察了肌动蛋白丝在活体状态下的运动和与膜的相互作用。katayma博士和toyoshima博士分别用云母薄片法和冰包埋电镜法描述了肌动蛋白细丝的分子结构。Drs。Owaribe和Tsukita介绍了作用结合蛋白的分子生物学数据。

Purpose of this project is to reveal the three dimensional dynamic structure of cytoskeleton or membrane skeleton related to the signal transduction. For this purpose, we are developing new imaging technology including atomic force microscope, energy filtering electron microscope and other improved video-microscope. Furthermore, this project is also dedicated a little to disclose the molecular structure of proteins, since signal transduction is based on molecular recognition of each related proteins. In other words, a series of molecular recognition is a cascade of signal transduction. Obviously, molecular-molecular recognition is involved in the shape or surface structure of molecules.Activity of individuals in this team.Project leader, Usukura made a report of this project, at the same time, he tried to image the transcription of DNA in this year. Dr.Sokabe revealed relationship between opening of mechanosensory channels and cytoskeletons by patch clump method combined with video-enhanced microscopy. Dr.Kusumi reported regulatory system of membrane proteins movement relating to the domain structure comprised of cytoskeletons beneath the membrane. Dr.Ishikawa revealed undercoat structure of the plasma membrane using confocal microscope. Dr.Kinoshita succeeded to observe actin filaments movement and interaction with the membrane in living state using new type of nanosecond multi-imaging microscope. Dr.Katayma and Dr.Toyoshima described molecular structures of actin filaments by mica flake method and ice embedding electron microscopic methods respectively. Drs.Owaribe and Tsukita presented molecular biological data of action binding proteins.